开发一种磁稳定流化床生物反应器,用于酶合成2 - 乙基烯酸酸盐
Mateus V C da Silva1, Amanda B S Rangel1, Cíntia M R Rosa1
1Department of Chemical Engineering, Engineering School of Lorena, University of São Paulo, Lorena, SP, 12602-810, Brazil.
Bioprocess and biosystems engineering
|October 10, 2023
概括
这项研究使用磁性生物催化剂开发了2-乙烯烯酸的连续酶合成. 优化的磁场强度提高了生产力,并证明了优异的生物催化剂稳定性,用于改进绿色化学过程.
科学领域:
- 生物催化和酶工程 生物催化和酶工程
- 绿色化学和可持续的过程
- 材料科学和纳米技术
背景情况:
- 酶合成为传统的化学方法提供了一个可持续的替代品,以生产.
- 磁支上的固定酶可以促进催化剂的回收和重复使用,这对于工业应用至关重要.
- 磁稳定流体床反应器 (MSFBRs) 在连续运行中提供了增强的质量转移和过程控制.
研究的目的:
- 开发和研究使用固定Candida antarctica脂酶的2-乙烯烯酸的连续合成.
- 在MSFBR中评估磁性聚 styrene-co-divinylbenzene (STY-DVB-M) 颗粒的性能.
- 优化反应条件,特别是磁场强度,以提高合成生产率和生物催化剂稳定性.
主要方法:
- 由STY-DVB-M颗粒上固定的脂酶催化的2-乙烯酸酸盐的合成.
- 使用FTIR,DSC和TGA对STY-DVB-M物理特性进行表征.
- 使用不同的磁场强度 (5-15mT) 连续运行MSFBR,并分析反应产量,度和生产率.
- 评估生物催化剂的运行稳定性和热失活动力学.
- 使用Scilab基于乒乓球双双运动的计算模拟和数学建模.
主要成果:
- 该STY-DVB-M支表现出有利的热性能 (Tg = 85.68°C,T_onset = 406.66°C).
- 在15mT磁场强度下,达到0.850 ± 0.023 mmol g−1 h−1的最大生产率.
- 生物催化剂表现出了显著的操作稳定性,半衰期为2148小时 (179个周期) 和较低的失活常数.
- 数学建模准确地预测了反应产量 (R2 = 0.926) 和间位速度.
结论:
- 使用磁性生物催化剂在MSFBR中连续合成2-乙烯酸酸盐是一种高效和稳定的过程.
- 优化的磁场强度显著提高了反应生产率.
- 这种方法代表了推进工业生物催化剂合成以提高可持续性的有希望的策略.
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