使用介电光谱来识别和分离可活的酵母细胞
Sakshin Bunthawin1, Paphawarin Srichan1, Kata Jaruwongrungsee2
1Biotechnology of Electromechanics Research Unit, Faculty of Technology and Environment, Prince of Songkla University, Kathu, Phuket, 83120, Thailand.
Applied microbiology and biotechnology
|October 10, 2023
概括
介电泳通过分析它们独特的介电性质,有效地将可活和不可活的酵母细胞分开. 这种方法提供了一个可扩展的解决方案,用于评估发酵工业中的细胞生存能力.
科学领域:
- 生物物理学的生物物理.
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 评估酵母细胞生存能力对于发酵和酒行业至关重要.
- 传统方法可能耗时,可能无法提供实时分析.
- 脱电泳 (DEP) 是一种潜在的非侵入性细胞分析方法.
研究的目的:
- 调查使用电介电泳来区分可活和不可活的酵母细胞.
- 分析酵母细胞的介电性质,以确定其生存能力.
- 评估DEP在工业发酵中的细胞分离潜力.
主要方法:
- 酵母细胞 (Saccharomyces cerevisiae) 使用微平行圆柱形电极对非均的正弦形电场进行了试验.
- 细胞活力通过分析不同介质导电性的二电电泳光谱和较低的临界频率 (LCF) 来评估.
- 实时图像处理和顺序信号发生器 (SSG) 用于监控细胞运动和收集产量.
主要成果:
- 活的和死的酵母细胞表现出不同的介电光谱和介电性质.
- 活酵母细胞的细胞质导电性 (σc) 约为0.2 S m−1,明显高于死细胞的导电性 (≤0.05 S m−1).
- 观察到正极电泳 (pDEP),LCF值根据介质导电性发生变化.
结论:
- 脱电泳是一种可行的方法,用于区分和分离可活的和不可活的酵母细胞.
- 细胞介电性质,特别是细胞质导电性,是酵母细胞活力和代谢健康的可靠指标.
- 该技术为发酵行业的质量控制提供了可扩展和实时的方法.
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