在玉米基因内插入突变型转子子素往往会提供一种新的向外阅读促进体
Erika L Ellison1, Peng Zhou1, Peter Hermanson1
1Department of Plant and Microbial Biology, University of Minnesota, Saint Paul, MN 55108, USA.
Genetics
|October 10, 2023
概括
玉米中的突变者 (Mu) 转位子可以通过向外阅读促进体激活基因表达. 这一发现提供了对转子子基因相互作用和玉米遗传学研究的见解.
科学领域:
- 植物分子生物学 植物分子生物学
- 遗传学 是一个遗传学.
- 基因组学就是基因组学.
背景情况:
- 突变者 (Mu) DNA转位子是玉米遗传研究的重要工具.
- Mu-抑制性等位基表现出依赖于Mu活动的条件表型.
- 表型抑制通常与Mu元素中的向外阅读促进体有关.
研究的目的:
- 为了研究Mu转子体促进体在基因表达中的作用.
- 从Mu插入等位基因生成的转录的特征.
- 了解Mu促进剂活性对玉米遗传学的影响.
主要方法:
- 从UniformMu玉米种群中分离了35 Mu等位基因.
- RNA 测序 (RNA-seq) 和 de novo 转录组装.
- 在Mu序列和基因表达模式内分析转录启动.
主要成果:
- 在33个等位基因中,有20个等位基因的转录在Mu序列中启动的证据.
- 在不同的Mu元素类型和方向中观察到Mu促进剂活性.
- 穆启动的转录通常模仿野生类型的基因表达水平和模式.
结论:
- 穆促销器作为一个最小的促销器,对cis-regulatory元素作出反应.
- Mu转位子可以通过促进体介导的基因调节与宿主基因组共存.
- 这些发现对研究人员在玉米研究中利用UniformMu种群的研究人员来说很重要.
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