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相关概念视频

RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Types of RNA01:20

Types of RNA

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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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RNA Editing02:23

RNA Editing

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Experimental RNAi02:15

Experimental RNAi

6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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相关实验视频

Updated: Jul 14, 2025

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Published on: December 9, 2020

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加勒-3与RNA没有直接相互作用.

Egan L Peltan1,2, Nicholas M Riley2,3, Ryan A Flynn4,5

  • 1Department of Chemical and Systems Biology, Stanford University School of Medicine, 269 Campus Drive CCSR 4145 Stanford, CA 94305, United States.

Glycobiology
|October 10, 2023
PubMed
概括
此摘要是机器生成的。

加勒-3不是一种直接的RNA结合蛋白. 研究表明,分离RNA-蛋白交叉链的抗体是由于hnRNPA2B1,而不是galectin-3本身.

关键词:
盖莱克蛋白 (Galectins) 是一种蛋白质.盖莱克-3 是一种RNA结合蛋白质是RNA结合的蛋白质.在 hnRNPA2B1B1 中.

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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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相关实验视频

Last Updated: Jul 14, 2025

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads
08:48

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells
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Desthiobiotin-Streptavidin-Affinity Mediated Purification of RNA-Interacting Proteins in Mesothelioma Cells

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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

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科学领域:

  • 分子生物学分子生物学
  • 葡萄糖生物学 葡萄糖生物学
  • 在RNA生物学,RNA生物学.

背景情况:

  • 已知一种糖甘结合蛋白的加勒-3被提出作为一种具有RNA结合能力的双重功能蛋白.
  • 这种假定作用表明了糖生物学和RNA生物学之间的联系,特别是在mRNA前拼接中.
  • 以前的证据依赖于缺乏遗传验证的亲和试剂,因此直接RNA相互作用未得到证实.

研究的目的:

  • 研究加勒-3的直接RNA结合能力.
  • 为了确定galectin-3是否在细胞过程中与RNA相互作用,如mRNA前成熟.
  • 澄清之前观察到的涉及加勒-3的RNA-蛋白关联的分子基础.

主要方法:

  • 使用抗体对抗内源性人体胆素-3用于RNA-蛋白交叉链接的免疫沉 (IP).
  • 对IP分离物进行了蛋白质组分析.
  • 产生了LGALS3淘汰细胞系和HNRNPA2B1淘汰细胞系.
  • 引入了LGALS3的内源C终端位置的表位标签,以进行有针对性的隔离.

主要成果:

  • 抗素-3抗体分离了RNA-蛋白交叉链接,但这是独立于LGALS3基因表达的.
  • 蛋白质组分析显示,RNA结合蛋白 hnRNPA2B1 在分离物中非常丰富.
  • 基因废除HNRNPA2B1,但不是LGALS3,废除了RNA-蛋白质交叉链路隔离.
  • 隔离带表位标记的 galectin-3 并没有产生RNA-蛋白质交叉链接.

结论:

  • 盖列-3似乎不会与RNA直接相互作用.
  • 观察到的RNA关联很可能是由于涉及hNRNPA2B1.1的交叉反应或间接相互作用.
  • 鉴定galectin-3为RNA结合蛋白可能是一个误解,特别是在HeLa细胞中.
  • 建议使用基因删除和内源性表位标签来精确评估蛋白质-RNA相互作用.