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追踪严重病情的成年人肠道微生物群和血液感染

Christopher H Gu¹, Layla A Khatib², Ayannah S Fitzgerald²

¹Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States of America.

|October 10, 2023

概括

肠道微生物组可以导致血液感染 (BSI),但确认这种联系需要精确的菌株识别. 测序显示,在许多情况下,血液中发现的肠道细菌与肠道中的细菌不是相同的菌株.

科学领域:

微生物学微生物学
基因组学就是基因组学.
传染性疾病传染性疾病

背景情况:

肠道微生物组通过细菌转移与血液感染 (BSI) 有关.
建立肠道和血液细菌之间的明确联系需要在菌株水平上进行鉴定.

研究的目的:

为了研究肠道微生物对危急病患者血流感染 (BSI) 的贡献.
应用全基因组测序来比较肠道和血液细菌的菌株水平.

主要方法:

肠道微生物组的枪式基因组测序和BSI分离物的全基因组测序.
肠道微生物组的调整读取到血液中,隔离基因组以评估菌株身份.
定义了序列身份的值,以区分相关和非相关的细菌菌株.

主要成果:

在50%的发作中,与BSI物种相匹配的细菌在肠道微生物群中被发现.
在14名具有匹配物种的可评估患者中,只有6人表现出肠道和血液之间的相同菌株.
一种物种在肠道中的较高相对丰度与血液分离物的序列相同性增加相关.

结论:

严格的,基于序列的方法对于确认肠道是BSI的来源至关重要.
在很大一部分情况下,血液中的细菌不是与肠道中的细菌相同的菌株.
全基因组测序是追踪患者感染源的关键工具.

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