了解YTHDF蛋白质分子功能的保护和多样化
Daniel Flores-Téllez1,2, Mathias Due Tankmar1, Sören von Bülow1
1University of Copenhagen, Biology Department. Copenhagen, Denmark.
PLoS genetics
|October 10, 2023
概括
这项研究揭示了m6A-YTHDF蛋白在刺激植物细胞分裂中的祖先功能在陆地植物中得到保护. 虽然所有阿拉比多普西斯ECT蛋白都与m6A结合,但一些通过其内在无序区域 (IDR) 的变化进化了新的功能.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- N6-甲基氨酸 (m6A) 是一个关键的mRNA修饰,具有YT521-B同源 (YTH) 域蛋白作为m6A读者.
- 在植物中,YTHDF类扩展为11种进化保存的C终端区域 (ECT) 蛋白质,功能多样化尚不清楚.
- 众所周知,ECT2,ECT3和ECT4可以冗余地刺激Arabidopsis的干细胞分裂.
研究的目的:
- 研究植物YTHDF (ECT) 蛋白的功能性保护和多样化.
- 为了确定不同的YTHDF蛋白是否可以执行刺激细胞分裂的祖先功能.
- 探索内在无序区域 (IDR) 在YTHDF蛋白功能中的作用.
主要方法:
- 使用ect2/ect3/ect4突变的Arabidopsis. 的功能补充试验.
- 测试各种YTHDF蛋白质拯救突变表型的能力.
- 分析IDRs的m6A结合能力和生物物理特性 (相位分离,电荷分布).
主要成果:
- 在刺激原始细胞分裂方面,m6A-YTHDF轴的祖先功能在陆地植物中得到保存,并且存在于所有主要的开花植物群中.
- 所有的阿拉比多普西斯ECT蛋白质都通过它们的YTH域具有m6A结合能力.
- 在ECT1,ECT9和ECT11中发生了谱系特定的新功能化,涉及改变的YTH域和IDR属性,特别是具有相隔倾向和电双极的IDR.
结论:
- 植物细胞分裂中的m6A-YTHDF蛋白质的核心功能是古老的和保存的.
- 植物中YTHDF蛋白的多样化涉及新功能化,特别是通过对内在无序区域的修改.
- 植物YTHDF中IDR的独特生物物理特性,与人类和中的不同,可能是物种特异性的分子活动的基础.
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