在Bacillus subtilis中,环境DNA的吸收通过动态的柱状结构发生在细胞表面的整个区域
Alexandra Kilb1, Marie Burghard-Schrod1, Sven Holtrup1
1Fachbereich Chemie und Zentrum für Synthetische Mikrobiologie, SYNMIKRO, Philipps-Universität Marburg, Marburg, Germany.
PLoS genetics
|October 10, 2023
概括
细菌细菌在能力发展过程中使用动态的柱状结构来吸收DNA. 外部DNA增加了柱子的参与,这表明有受调节的吸收机制.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 细菌细菌经历了DNA吸收能力的发展.
- DNA吸收对于遗传转变和适应至关重要.
研究的目的:
- 为了阐明初始DNA传输在Bacillus subtilis的细胞外的机制.
- 调查柱状结构和柱状动态在DNA吸收中的作用.
主要方法:
- 在活生生的 Bacillus subtilis 细胞中,单个分子水平研究主要的皮林,ComGC.
- 对柱子组装,收缩和柱子定位动态的观察.
- 在添加外部DNA时,分析柱状分布的变化.
主要成果:
- 一个动态的柱状结构,大约500纳米长,介导DNA穿越细胞壁的运输.
- 在细胞膜内的扩散和静止种群中存在ComGCpilin.
- 外部DNA增加了静止的ComGC的比例,表明增强了柱子的参与.
结论:
- 在DNA的存在下,pilus会随机组装,但会积极招募pilins.
- 这种动态的柱子系统有助于通过细胞外有效地吸收环境DNA.
- 提出了一个模型,DNA通过动态柱体穿过细胞表面进行吸收.
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