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Updated: Jul 14, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
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可切割的交叉连接器被新型MS-Trigger算法重新定义

Lars Kolbowski1, Lutz Fischer1, Juri Rappsilber1,2,3

  • 1Technische Universität Berlin, Chair of Bioanalytics, 10623 Berlin, Germany.

Analytical chemistry
|October 10, 2023
PubMed
概括

一个新的算法通过增强MS3触发分析来识别交叉链接,显著改善交叉链接质谱 (MS),提高结构系统生物学中的灵敏度和特异性.

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Conservative Site-specific Recombination and Phase Variation02:53

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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