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Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Updated: Jul 14, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

可切割的交叉连接器被新型MS-Trigger算法重新定义

Lars Kolbowski¹, Lutz Fischer¹, Juri Rappsilber^1,2,3

¹Technische Universität Berlin, Chair of Bioanalytics, 10623 Berlin, Germany.

Analytical chemistry

|October 10, 2023

概括

此摘要是机器生成的。

一个新的算法通过增强MS3触发分析来识别交叉链接,显著改善交叉链接质谱 (MS),提高结构系统生物学中的灵敏度和特异性.

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Synthesis of Programmable Main-chain Liquid-crystalline Elastomers Using a Two-stage Thiol-acrylate Reaction

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

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Synthesis of Programmable Main-chain Liquid-crystalline Elastomers Using a Two-stage Thiol-acrylate Reaction

Synthesis of Programmable Main-chain Liquid-crystalline Elastomers Using a Two-stage Thiol-acrylate Reaction

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科学领域:

结构生物学是结构生物学.
系统生物学系统生物学
质谱测量质量谱测量

背景情况:

交联质谱 (MS) 正在从结构生物学发展到系统生物学.
可切割MS的交叉链接器允许基于MS3的交叉链接的识别,减少了搜索空间.
目前基于MS2 (MS/MS) 的方法比基于MS3的方法更敏感.

研究的目的:

为了解决MS3中的算法限制,触发灵敏度和特异性.
为基于MS3的交叉链接鉴定开发一个改进的算法.

主要方法:

开发一种新的四步MS3触发算法.
与现有的MS3触发方法进行比较分析.

主要成果:

四步MS3触发算法显示显著提高了性能.
该算法的灵敏度和特异性接近了理论限制.
性能优于目前使用的基于MS3的方法.

结论:

开发的算法增强了基于MS3的交叉链接质谱的实用性.
这一进步有助于对交联的更敏感和更具体的识别.
能够实现更强大的结构系统生物学应用.