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每天的热变异性不会改变长期基因表达相对于稳定的热环境:一个热带鱼的案例研究

Hanna Scheuffele¹, Erica V Todd¹, John A Donald²

¹School of Life and Environmental Sciences, Deakin University, Geelong, VIC 3216, Australia.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

|October 10, 2023

概括

全球变暖对热带生物产生影响,其温度波动. 适应循环温度的巴拉蒙迪表现出与恒温相似的新陈代谢和生长基因调节,但增加了热冲击反应.

科学领域:

环境科学环境科学
分子生物学分子生物学
生态生态学生态生态学

背景情况:

全球变暖增加了极端天气事件和温度变化.
与恒温相比,对温度波动的ectotherm反应的了解很少.
了解热变性对于预测气候变化对热的影响至关重要.

研究的目的:

为了研究恒定和循环温度对巴拉蒙迪 (Lates calcarifer) 基因表达和表现的影响.
为了确定日间热变化是否会改变分子反应,与恒定的热环境相比.
评估热适应对新陈代谢,生长和细胞平衡基因的影响.

主要方法:

巴拉蒙迪已经适应了恒温 (23°C,29°C,35°C) 和循环温度 (23-35°C每天,平均29°C).
肝脏和白肌肉组织在2周和17周后取样,用于基因表达分析.
在NanoString nCounter技术测量了20个与新陈代谢,生长和细胞平衡相关的基因的表达.

主要成果:

适应恒定的冷和温暖的温度预计改变了整个动物的表现和基因表达.
与恒定29°C相比,适应循环温度并没有显著改变新陈代谢或生长基因调节.
在适应循环温度的barramundi中观察到更高的热冲击反应,表明对细胞监护的需求增加.

结论:

巴拉蒙迪的新陈代谢和生长的遗传调节似乎更多地取决于每日平均温度,而不是每日温度范围.
每天的温度波动可能需要增强细胞监护机制以减轻蛋白质损伤.
预测气候变化影响的现有模型可能需要考虑热变异对ectotherm生理学的差异影响.

关键词:

巴拉蒙迪·巴拉蒙迪 (Barramundi) 是一个鱼的生长鱼的生长鱼的生长拉特斯卡尔卡里费尔 (lates calcarifer) 是一种较为简单的葡萄酒.代谢过程中的代谢.纳米弦 n 计数器 n 计数器适应温度适应温度的过程.

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