蚊子的转子子介导转基因发生
Vanessa Bottino-Rojas1, Anthony A James2,3
1Department of Microbiology and Molecular Genetics, University of California, Irvine, California 92697-4500, USA.
Cold Spring Harbor protocols
|October 10, 2023
概括
新的可转移元素使得在携带疾病的蚊子中实现了常规基因工程. 多功能 piggyBac 元素在 Aedes,Anopheles 和 Culex 属中具有功能,用于医疗和研究应用.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 转子子介导的转基因对于疾病载体的基因工程至关重要.
- 使用Drosophila melanogaster的P元素的早期尝试在蚊子中没有成功.
- 开发新的可转移元素对于常规的蚊子转基因是必要的.
研究的目的:
- 审查蚊子载体中转子子介导转基因的进展.
- 为突出发现和验证用于蚊子研究的新型可转移元素.
- 讨论这些工具在理解基因功能和开发医疗干预方面的应用.
主要方法:
- 在蚊子物种 (Aedes,Anopheles,Culex) 中识别和验证新的可移植元素.
- 使用PiggyBac可转换元件,它在蚊子属和其他昆虫类中显示了广泛的功能.
- 转子子系统的应用,用于引入标记基因,记者系统和外源DNA.
主要成果:
- 在多个蚊子属中成功和可重复的转基因.
- 展示了piggyBac元素在各种昆虫物种中的广泛实用性.
- 建立用于定义基因控制序列和通过增强器捕获识别内源基因的方法.
结论:
- 在关键的蚊子载体中成功建立了转子介导的转基因.
- PiggyBac元素是用于蚊子和其他昆虫的基因操纵的多功能工具.
- 这些进展有助于研究疾病传播和开发新型控制策略.
更多相关视频
07:25Transfection and Mutagenesis of Target Genes in Mosquito Cells by Locked Nucleic Acid-modified Oligonucleotides
Published on: December 26, 2010
10.5K
09:45Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata
Published on: October 1, 2016
9.1K
相关概念视频
DNA-only Transposons
14.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
14.5K
Transposons
39
Transposons, or "jumping genes," are small mobile genetic elements (MGEs) that range from 700 to 40,000 base pairs in length. They are found in all organisms and can move within the same chromosome or transfer to different chromosomes. In some cases, transposons can also jump between different host DNA molecules, such as plasmids or viruses, contributing to genetic variability.Barbara McClintock first discovered these mobile genetic elements in the 1940s while studying maize genetics, and she...
39
Overview of Transposition and Recombination
15.6K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
15.6K
Transgenic Organisms
31.2K
Overview
31.2K
