相关实验视频
Updated: Jul 14, 2025

10:40
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
Published on: April 25, 2022
2.4K
对circRNA表达特征和circRNA-miRNA-mRNA网络对非常早期发病的精神分裂症敏感性的全面分析
Huanhuan Huang1, Jie Luo1, Yanjie Qi1
1National Clinical Research Center for Mental Disorders, Beijing Key Laboratory of Mental Disorders, Beijing Anding Hospital, Beijing Institute for Brain Disorders Capital Medical University, Beijing, People's Republic of China.
Schizophrenia (Heidelberg, Germany)
|October 10, 2023
概括
这项研究确定了循环RNAs (circRNAs) 作为儿童早期精神分裂症 (VEOS) 的潜在生物标志物. 在VEOS患者中较低的circRNA水平表明它们在疾病发展中的作用,并提供新的诊断可能性.
科学领域:
- 遗传学和基因组学 遗传学和基因组学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 非常早期发作的精神分裂症 (VEOS) 是一种严重的精神疾病,对其潜在的分子机制的理解有限.
- 循环RNAs (circRNAs) 正在成为各种生物过程和疾病的关键调节者,但它们在VEOS中的作用仍然未被探索.
研究的目的:
- 与健康对照组相比,研究VEOS儿童血中circRNAs,microRNAs (miRNAs) 和信使RNAs (mRNAs) 的差异表达.
- 构建一个circRNA-miRNA-mRNA (ceRNA) 网络,并预测circRNAs在VEOS病变发生中的功能作用.
主要方法:
- 来自10名VEOS患者和8名健康对照者的血样本使用RNA测序 (RNA-seq) 来分析circRNAs,miRNAs和mRNAs.
- 生物信息工具 (miRanda) 用于预测miRNA-目标相互作用并构建一个ceRNA网络.
- 基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 路径分析在目标mRNAs上进行.
主要成果:
- 1934年确定了circRNAs,与对照组相比,VEOS患者的表达水平明显较低.
- 不同表达分析显示了235个circRNAs,11个miRNAs和2,308个mRNAs,其中VEOS有显著的变化.
- 构建了一个ceRNA网络,包括10个下调节的circRNA,6个上调节的miRNA和47个下调节的mRNA,涉及与膜,信号转导和细胞骨相关的途径.
结论:
- 这项研究是首次报告VEOS患者血中差异表达的circRNAs,突出了它们作为诊断生物标志物的潜力.
- 构建的circRNA-miRNA-mRNA网络为VEOS中circRNAs的调节机制提供了洞察力.
- 在这项研究中发现的circRNAs可能在VEOS症状的出现和进展中发挥关键作用.
相关概念视频
Biological Causes of Schizophrenia
64
Schizophrenia, a severe psychiatric disorder, arises from a complex interplay of biological factors, including genetic predisposition, structural brain abnormalities, neurotransmitter dysregulation, and developmental irregularities. These factors collectively contribute to the onset and progression of the disorder, which typically manifests in late adolescence or early adulthood.
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
Genetic Factors in Schizophrenia
The genetic basis of schizophrenia is strongly supported by family and twin...
64
MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Psychological and Sociocultural Causes of Schizophrenia
95
Schizophrenia, a complex psychiatric disorder, has been historically misunderstood. Early psychological theories attributed its origins to childhood trauma and unresponsive parenting. However, contemporary research largely rejects these notions, favoring the vulnerability-stress hypothesis. This model proposes that individuals with a genetic predisposition to schizophrenia may develop the disorder following exposure to significant environmental stressors. Notably, studies on high-risk...
95
Psychosis: Pathophysiology of Schizophrenia and Other Psychotic Disorders
700
Schizophrenia is a neurodevelopmental disorder whose origins are rooted in complex genetic components. Despite our burgeoning understanding, the pathophysiology of this disorder remains incompletely deciphered.
Researchers have identified genetic factors that increase susceptibility to schizophrenia, underscoring the intricate interplay between genetics and environment in disease development. At the core of schizophrenia's pathophysiology is excessive dopaminergic neurotransmission within...
Researchers have identified genetic factors that increase susceptibility to schizophrenia, underscoring the intricate interplay between genetics and environment in disease development. At the core of schizophrenia's pathophysiology is excessive dopaminergic neurotransmission within...
700
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K

