坎皮洛巴克特 (Campylobacter jejuni) 的基因定型及其抗菌素耐药性的预测工具
Nicol Strakova1, Hana Michova2, Ekaterina Shagieva2
1Veterinary Research Institute, Hudcova 296/70, Brno, Czech Republic. nicol.strakova@vri.cz.
Folia microbiologica
|October 10, 2023
概括
多部位序列类型 (MLST) 是对Campylobacter jejuni的基因型定型的最佳工具,Campylobacter jejuni是导致食物传播疾病的常见原因. 与其他技术相比,这种方法提供了高精度,成本效益和效率.
科学领域:
- 微生物学 微生物学
- 食品安全 食品安全
- 遗传学 遗传学 是一个
背景情况:
- 坎比洛巴克特 (Campylobacter jejuni) 是导致食物传播疾病的主要原因,但其基因组的变异性使源追踪复杂化.
- 有效的基因型定型方法对于了解C. jejuni的传播动态至关重要.
研究的目的:
- 为了比较三种基因定型方法 (MLST,PFGE,mP-BIT) 对C. jejuni.的有效性.
- 评估抗微生物耐药性预测工具与C. jejuni.的表型敏感性测试进行对比.
主要方法:
- 与cgMLST参考数据对MLST,PFGE和mP-BIT进行比较分析.
- 基因定型方法的成本效益分析.
- 基于ResFinder和RGI基因的预测与抗菌素耐药性磁盘扩散的比较.
主要成果:
- MLST表现出最高的反向歧视指数 (97%) 和优越的成本效益概况.
- PFGE和mP-BIT分别显示了歧视权和分离权的限制.
- ResFinder和RGI准确地预测了抗菌素耐药性,与光盘扩散结果保持一致.
结论:
- 由于其效率和准确性,MLST是C. jejuni基因定型的最佳工具.
- 基于基因的预测工具 (ResFinder,RGI) 可靠地评估C. jejuni的抗菌耐药性.
关键词:
抗微生物耐药性 抗微生物耐药性在MLST上,我们可以看到MLST.现在的PFGE就是PFGE.地区原产地地标.在ResFinder中,我们可以使用ResFinder.cgMLST 的意思mP-BIT 的使用.更多相关视频
05:34Author Spotlight: Development of an Enhanced Protocol for Rapid and Accurate Isolation of Campylobacter from Food Products
Published on: February 23, 2024
1.9K
08:23Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens
Published on: March 10, 2020
11.6K
相关概念视频
Development of Antibiotic Resistance
19
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
19
Antibiotic Selection
54.5K
Overview
54.5K
Modern Molecular Taxonomy
26
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
26
