宏细胞向的氧化铁纳米诱增强了先天免疫和药物杀伤,以获得更有效的Mycobacterium Tuberculosis清除
Ling Shen1, Kangsheng Liao2,3,4, Enzhuo Yang5,6
1Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, IL, USA. lshen@uic.edu.
Journal of nanobiotechnology
|October 10, 2023
概括
这项研究引入了一种新的氧化铁纳米粒子 (IONP) 纳米诱,用于对抗结核病 (TB). 这种纳米诱可增强药物输送,并增强免疫系统,有效地减少感染细胞和小鼠中的Mycobacterium结核病 (Mtb).
科学领域:
- 纳米医学是一种纳米医学.
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 由Mycobacterium tuberculosis (Mtb) 引起的结核病 (TB) 仍然是全球领先的传染病杀手.
- 结核病控制的挑战包括mtb的免疫逃避和药物的有效性有限.
- 铁对Mtb是必不可少的,但在高剂量时是有毒的,呈现出治疗点.
研究的目的:
- 设计和评估以铁氧化物纳米颗粒 (IONP) 为基础的以巨为目标的纳米诱 (IONPs-PAA-PEG-MAN) 进行增强的抗结核治疗.
- 为了研究纳米诱增强先天免疫力和药物诱导的细胞内Mtb.杀戮的能力.
- 评估在体外,体外和体内含有利芬素的纳米诱惑剂 (Rif@IONPs-PAA-PEG-MAN) 的治疗潜力.
主要方法:
- IONPs-PAA-PEG-MAN纳米诱的合成和表征.
- 评估巨细胞吸收和药物输送效率.
- 在受感染的巨细胞和单细胞衍生巨细胞中杀死Mtb的评估.
- 在感染Mtb的小鼠体内研究,以评估疗效和病理学.
主要成果:
- 纳米诱被巨细胞优先吸收,从而增加了宿主细胞内的药物度.
- Rif@IONPs-PAA-PEG-MAN促进了Mtb菌体-纳米诱在溶酶体中的共同定位,增强了药物释放和杀死Mtb.
- 纳米诱使Mtb感染的巨细胞偏向抗菌菌M1表型,增加TNF-α的产生.
- 在接受治疗的小鼠中观察到肺部菌群负荷的显著减少和病理的缓解.
结论:
- Rif@IONPs-PAA-PEG-MAN纳米诱通过联合免疫调节和改善药物输送,协同增强细胞内MTB杀死.
- 这种纳米诱策略对开发更有效的结核病治疗方法充满希望.
- 这些发现强调了向纳米医学在克服结核病治疗方面的挑战方面的潜力.
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