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在KBTBD6位点的印记涉及特定物种的母体甲基化和畜牧动物的单基因表达
Jinsoo Ahn1, In-Sul Hwang2,3, Mi-Ryung Park2
1Department of Animal Sciences, The Ohio State University, Columbus, OH, 43210, USA.
Journal of animal science and biotechnology
|October 11, 2023
概括
KBTBD6基因的基因印记是特定于养哺乳动物,不同于人类和小鼠. 这种特定于牲畜的印记表明基因调节中的进化分歧.
科学领域:
- 基因组学就是基因组学.
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 哺乳动物发展 哺乳动物发展
背景情况:
- 不同甲基化区域 (DMRs) 控制印记基因表达,对哺乳动物发育至关重要.
- 识别特定物种的印记基因需要先进的比较方法.
- 研究了KBTBD6位点的跨物种印记模式.
研究的目的:
- 检查跨物种和组织的KBTBD6基因的DNA甲基化和等位基因表达.
- 探索驱动 KBTBD6 印记的潜在机制.
- 为了识别特定物种的印记基因.
主要方法:
- 在猪胚胎上进行了全基因组双硫酸盐测序和RNA测序.
- 在KBTBD6中,在各种物种和组织中分析了DNA甲基化和基表达.
- 在转录中的CpG岛甲基化在卵细胞中进行了研究.
主要成果:
- 在猪胚胎中鉴定出KBTBD6促进体的母性超甲基化DMR,导致父单基表达.
- KBTBD6表现出特定于牲畜的印记,在卵细胞中甲基化/组织中的基表达,与人类,非人类灵长类动物和小鼠不同.
- 猪和牛卵细胞中转录的CpG岛屿,以及活跃的转录标记和DNA甲基化,表明转录依赖的印记.
结论:
- KBTBD6基因在养哺乳动物中显示印记表达,但在人类,非人类灵长类动物或小鼠中并非如此.
- 这项比较研究强调了基因组印记的物种特异性演变.
- 这些发现有助于理解哺乳动物间印记机制的多样性.
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