一种新型的Brevinin2 HYba5抗Staphylococcus aureus和Enterococcus faecalis的多微生物生物膜

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Megha Periyappilly Radhakrishnan¹, Karthika Suryaletha¹, Iype Joseph¹

¹Cholera & Biofilm Research Laboratory, Pathogen Biology Group, Rajiv Gandhi Centre for Biotechnology, Govt. of India, Trivandrum, 695 014, Kerala, India.

Protein and peptide letters

|October 11, 2023

概括

布雷维宁2 HYba5有效抑制金黄色葡萄球菌和菌菌的生物膜,显示了与设备相关的感染管理的希望. 这种新型化合物为抗生素提供了潜在的替代品,用于对抗持续性细菌感染.

科学领域:

抗微生物是一种抗微生物.
生物膜研究生物膜研究
传染性疾病传染性疾病

背景情况:

布雷维宁2 HYba5 (酸二九) 是一种来自*Hydrophylax bahuvistara*的新型阴阳性酸.
黄金葡萄球菌 (Staphylococcus aureus) 和菌 (Enterococcus faecalis) 形成麻烦的生物膜,导致抗生素耐药性和持续性感染.
需要针对多种生物膜的新型,稳定且毒性较低的化合物.

研究的目的:

为了评估Brevinin2 HYba5对*S. aureus*和*E. faecalis*混合生物膜的活性.

主要方法:

使用水晶紫色测定,共聚焦激光扫描显微镜 (CLSM) 和MTT测定来评估抗生物膜活性.
用RBC和L929纤维细胞细胞系来评估细胞毒性.
生物膜抑制在不同温度,pH值,血清和血度下进行了测试,并使用光 *in situ* 杂交 (FISH) 和盘子计数对抗多微生物生物膜.

主要成果:

29抑制了*S. aureus*和*E. faecalis*生物膜的形成,单独和在75μM的多微生物环境中.
该在不同的温度,血清和血度中保持了抗菌膜活性.
活性在酸性和中性pH下是最佳的,在性pH下有效性降低. 该表明非溶血性和低细胞毒性.

结论:

布雷维宁2 HYba5 具有显著的生物膜抑制特性,可以对抗 *S. aureus* 和 *E. faecalis*.
29是治疗生物膜相关感染的有希望的候选物,特别是在导管相关器件中.
它的潜力在于防止最初的病原体殖民,从而减少感染负担.

关键词:

抗生物膜活动活性.菌 (Enterococcus faecalis) 是一种菌.黄金葡萄球菌黄金葡萄球菌布雷维宁2 HYba5 类感染. 感染.聚微生物生物膜是一种多微生物生物膜.

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