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阿格林是一种在甲状腺癌中使用的新型致癌蛋白
Anna Adamiok-Ostrowska1, Małgorzata Grzanka1, Barbara Czarnocka1
1Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical Education, 01-813 Warsaw, Poland.
Oncology letters
|October 11, 2023
概括
增加的阿格林 (AGRN) 表达促进甲状腺癌的进展和免疫透. 沉默AGRN降低了癌细胞活力,迁移和入侵,这表明AGRN是潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 亚格林 (AGRN) 是一种母细胞糖蛋白,与瘤发生和癌症进展有关.
- AGRN在甲状腺癌 (TC) 发病过程中的特定作用尚不清楚.
研究的目的:
- 调查AGRN在甲状腺癌 (TC) 发展和进展中的作用.
- 分析各种TC亚型和细胞系中的AGRN表达水平.
- 评估AGRN对TC细胞行为的影响及其与免疫透的关联.
主要方法:
- 在TC细胞系 (CGTH,FTC-133,BcPAP) 和患者转录组数据中分析AGRN表达.
- 试管测试包括迁移,入侵,生存能力和扩散测试.
- 对表皮细胞-介质细胞过渡 (EMT) 标记物和对AGRN定位的免疫细胞化学进行西部斑分析.
主要成果:
- 在甲状腺瘤和TC细胞系中,AGRN表达显著升高,特别是在毛囊和乳头亚型中.
- 在正常细胞和TC细胞中观察到AGRN的核定位.
- 沉默AGRN降低了TC细胞活力,增殖,迁移和入侵,伴随着EMT标记物的改变表达 (vimentin,N-cadherin,E-cadherin).
- 增加的AGRN表达与增加的甲状腺瘤中中性粒细胞透相关.
结论:
- 增加AGRN表达促进甲状腺癌细胞中的瘤原体表型.
- AGRN表达与甲状腺瘤中的免疫透有关.
- AGRN代表了甲状腺癌的潜在治疗标,需要进一步调查.
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