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银纳米颗粒的生物制造使用Nostoc肌肉Lukesova 2/91:优化,表征和生物应用

Reham Samir Hamida¹, Mohamed Abdelaal Ali², Fatima Tariq Sharif³

¹Institute for Protein Research, Osaka University, Osaka, 565-0871, Japan.

International journal of nanomedicine

|October 11, 2023

概括

这项研究表明,Nostoc muscorum Lukesova 2/91可以有效地生物合成小,稳定的银纳米粒子 (AgNPs). 这些新型纳米粒子具有显著的抗癌和抗微生物特性,具有环保的治疗潜力.

科学领域:

生物技术和纳米技术
微生物学微生物学
材料科学材料科学材料科学

背景情况:

纳米粒子 (NP) 的生物合成为化学方法提供了一个环保,可扩展和生物相容的替代方案.
菌,如 *Nostoc muscorum *,是NP生物合成的有希望的微生物,因为它们的广泛性和已被证明的生物工厂的有效性.
发现新的微藻菌株来产生小,稳定的NP仍然是一个关键的研究领域.

研究的目的:

为了隔离和描述 *Nostoc muscorum* Lukesova 2/91 作为纳米粒子生物合成的潜在来源.
使用 *N. muscorum* 藻类提取物优化银纳米颗粒 (AgNPs) 生物合成条件.
评估生物合成的银纳米颗粒的抗增殖和抗微生物活性.

主要方法:

通过显微镜和DNA测序,将 *Nostoc muscorum* Lukesova 2/91 分离,净化和遗传鉴定.
在藻类提取物中分析挥发性生物分子,使用气色谱-质谱 (GC-MS).
使用UV-VIS,FTIR,TEM,SEM,EDx,映射和Zetasizer进行银纳米粒子 (Nos@AgNPs) 的生物合成和表征.

主要成果:

诺斯@AgNPs生产的最佳条件是:1毫米的AgNO3,1:1的提取物比率,25°C,光,24小时,pH7.4.4.
特性No@AgNPs:波长为401.4纳米,立方到圆形,平均直径为11.8±0.5纳米,含银量为86.15%,电荷为19.7mV.
FTIR分析表明,蛋白质/多糖类对NP合成起到减少剂和稳定剂的作用.

结论:

*Nostoc muscorum* Lukesova 2/91是一种有效的生物制造小,稳定的银纳米颗粒的来源.
生物合成的Nos@AgNPs显示出对肺癌,结肠癌和乳腺癌细胞系的显著抑制活性.
诺斯@AgNPs对关键的致病细菌具有相当大的生物杀伤活性,包括金黄色葡萄球菌和大肠杆菌.

关键词:

诺斯托克公司Nostoc sp.藻类是一种藻类.这是一种抗癌药物.抗微生物药物是一种抗菌药物.抗氧化剂是一种抗氧化剂.绿色合成是一种绿色合成.

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