对参与"夏季黑色"葡萄中paradormant芽释放反应的基因进行比较转录组分析
Shaogang Fan1, Feixiong Luo1, Meijun Wang1
1College of Horticulture, Hunan Agricultural University, Changsha, Hunan, China.
Frontiers in plant science
|October 11, 2023
概括
葡萄树的冬季芽,为半年收获而发芽,是由休眠状态调节的. 短修剪通过改变顶峰主导度释放了paradormancy,而胺通过破坏线粒体功能来打破了endodormancy.
科学领域:
- 植物生理学 植物生理学
- 园艺园艺 园艺园艺
- 分子生物学分子生物学
背景情况:
- 葡萄藤种植依赖于冬季的芽生长为半年收获,涉及连续的休眠阶段:paradormancy,endodormancy和ecodormancy.
- 内臭释放产生第一个作物,而内臭释放使第二次收获成为可能.
- 胺破坏了内臭性,抵消了ABA,用H2O2和乙烯作为信号分子.
研究的目的:
- 研究短修剪和胺处理释放葡萄藤冬季芽的机制.
- 澄清顶点主导在抑制paradormant芽生长中的作用.
- 阐明涉及休眠释放的分子途径.
主要方法:
- 葡萄藤芽的比较转录组分析.
- 农业处理,包括短修剪和胺的应用.
- 分析与辅酶运输,活性氧物种 (ROS) 和信号通路相关的基因表达.
主要成果:
- 简短的修剪有效地释放了paradormancy直到8月,突出了顶峰主导的抑制作用.
- 转录基因组分析显示,短修剪诱导了顶端组织转化和通过上调的VvPIN3和VvTIR1.1通过极极auxin运输.
- 简短的修剪刺激了ROS生成,通过MAPK级联中伤害,乙烯和H2O2放大信号.
- 胺破坏了线粒体的功能,导致ROS的产生和长时间的生长激素信号通路的诱导.
结论:
- 短修剪通过调节顶主导和auxin运输来释放paradormancy,同时还激活ROS介导的信号.
- 胺通过直接影响线粒体功能和激素信号传递来促进芽的生长,为休眠释放提供了独特的机制.
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