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细胞外无机酸盐的增加抑制了乳腺癌细胞中的ecto-phosphatase活性:通过过氧化进行调节

Marco A Lacerda-Abreu¹, José R Meyer-Fernandes¹

¹Instituto de Bioquímica Médica Leopoldo De Meis, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Cell biology international

|October 11, 2023

概括

高细胞外酸盐水平通过诱导氧化应激而降低了ecto-phosphatase活动. 由酸盐产生的过氧化 (H2O2) 抑制了ecto-phosphatase,影响了瘤微环境调节.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
在瘤学瘤学.

背景情况:

血膜上的ectoenzymes水解化分子,以使细胞吸收无机酸盐 (Pi).
细胞外酸酶活动对于调节细胞外Pi水平至关重要.

研究的目的:

为了研究细胞外Pi的增加对ecto-phosphatase活性的影响.
阐明Pi诱导的ecto-phosphatase抑制的机制.
探索对瘤微环境的影响.

主要方法:

在不同的Pi度下评估ecto-phosphatase活性.
测量过氧化 (H2O2) 的产生.
研究氧化应激和脂质过氧化在酶抑制中的作用.
检查H2O2介导抑制的可逆性.

主要成果:

细胞外Pi水平的升高会导致细胞外酸酶活性降低.
Pi诱导的氧化应激,特别是H2O2生成,是抑制的原因.
通过脂质过氧化,H2O2可以选择性地抑制酸铁的水解.
通过H2O2抑制ecto-酸酶是不可逆转的.
这种机制起到调节反的作用,防止细胞外Pi的过度积累.

结论:

高的细胞外Pi触发了通过H2O2诱导的氧化应激对ecto-phosphatase活动的不可逆转的抑制反循环.
这种调节对于管理Pi平衡至关重要,特别是在瘤微环境中.
这些发现为癌症等病理疾病中的ecto-phosphatase调节提供了新的见解.

关键词:

在MCF-7中使用.在MDA-MB-231中使用.酸酸酶是一种酸性酸酶.通过ecto-酸酶的活性.无机酸盐是一种无机酸盐.

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