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有效的3'-配对使得microRNA向对mRNA种子可访问性的敏感性降低

David M Kosek¹, Elnaz Banijamali², Walter Becker²

¹Department of Cell and Molecular Biology, Karolinska Institute, Biomedicum 9B, Solnavägen 9, 17177Stockholm, Sweden.

Nucleic acids research

|October 11, 2023

概括

微RNA (miRNA) 使用3'-配对来准mRNA,即使种子区域无法访问. 这种相互作用提高了基因沉默效率,并改善了miRNA目标预测模型.

科学领域:

分子生物学分子生物学
遗传学遗传学是一个
生物化学生物化学

背景情况:

微RNAs (miRNAs) 在转录后调节基因表达.
微RNA点识别主要涉及种子区域 (核酸2-8).
在种子区域之外的3 - 配对也影响了miRNA:目标相互作用.

研究的目的:

研究3配对和mRNA二次结构对miRNA抑制效率的综合影响.
提供结构性和功能性证据,证明3-配对在克服种子结合场所难以进入方面的作用.
为了完善有效的miRNA目标部位的预测.

主要方法:

选择性2 - 酸乙化分析通过原料扩展 (SHAPE) 来研究RNA结构.
路西法酶记者测定测量基因抑制效率.
对3个未翻译区域 (3-UTR) 的转录组学分析和高通量建模.

主要成果:

3 - 配对可以补偿无法访问的种子结合部位,从而实现miRNA介导的抑制.
miRNA 3 配对可以与种子部位上游的 mRNA 序列相互作用.
目标部位的头发针和凸起是可以容忍的,SHAPE证实了有效的3对配对预测的结构.
像PHB2 (miR-141) 这样的难以接近的目标可以通过3 - 配对有效地抑制.

结论:

3 - 配对是 miRNA 目标识别的一个重要因素,特别是在种子可访问性较低的网站.
这项研究提供了一个精细的模型来预测miRNA标的有效性.
了解3-配对增强了我们对miRNAs转录后基因调节的知识.

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