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Photoluminescence: Applications

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Updated: Jul 13, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

照亮分裂的阿普坦:用于传感的结合热力学和动力学.

Yichen Zhao¹, Nikesh Patel^1,2, Peihuan Sun¹

¹Department of Chemistry, Waterloo Institute for Nanotechnology, Waterloo, Ontario, N2L 3G1, Canada. liujw@uwaterloo.ca.

|October 11, 2023

概括

分开的阿巴特马保留了四环素抗生素结合功能,较长的茎长度与全长的阿巴特马具有相似的亲和力. 较短的茎增加了解离常数,影响了生物传感器的性能.

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相关实验视频

Last Updated: Jul 13, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers

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Published on: August 9, 2022

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

科学领域:

生物化学生物化学
分子生物学分子生物学
生物传感器技术技术

背景情况:

胺是DNA或RNA分子,具有很高的特异性来结合目标.
分裂的阿帕特马虽然在生物传感器中使用,但缺乏基本的结合研究.
对生物传感器的发展至关重要的是了解分裂的阿普坦酶结合.

研究的目的:

为了研究与全长体相比,分裂体的结合特性.
为了评估茎长度对分离的阿普坦酶结合亲和力和稳定性的影响.
开发一种新型的色度生物传感器,利用分裂的体.

主要方法:

设计和合成具有不同茎长度的分裂体.
光增强试验用于测量结合亲和力 (Kd).
温度依赖的光和异热定位热量测量 (ITC) 用于稳定性和热力学分析.
使用金纳米颗粒和硫化胺体的色度测试开发.

主要成果:

较长的茎分裂体表现出与全长体相比的解离常数 (Kd).
较短的茎分裂体显示Kd增加了85倍,表明结合亲和力减少.
分裂的阿普坦体显示出较低的热稳定性.
ITC 显示,在分裂的阿普坦酶结合后,热量释放增加,损失更大.
一个功能性的色度生物传感器成功地使用预装成的分开的阿帕特马半体成功开发出来.

结论:

分裂的阿帕特马干长度显著影响结合亲和力和稳定性.
分裂的aptamers为生物传感器开发提供了一个可行的平台,需要仔细考虑设计.
开发的色度生物传感器展示了分裂的阿巴特默在检测四环素时的实际应用.