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环境和临床菌株的广泛基因组测序和基因型鉴定

Alfonso Esposito¹, Silvano Piazza²

¹Faculty of Medicine and Surgery, "Kore" University of Enna, Enna, Italy. alfonso.esposito@unikore.it.

Methods in molecular biology (Clifton, N.J.)

|October 11, 2023

概括

下一代测序 (NGS) 已经彻底改变了Pseudomonas aeruginosa基因组学,使广泛的数据分析成为可能. 这项工作详细介绍了NGS方法和生物信息学工具,用于基因型推断和可视化.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

下一代测序 (NGS) 的出现显著推进了对Pseudomonas aeruginosa基因组学的研究.
现在有超过5700个P. aeruginosa基因组公开提供,推动了复杂的生物信息学工具的开发.
生物信息学已经成为管理和解释大规模基因组和表型数据的关键.

研究的目的:

为提供P. aeruginosa基因组测序发现的历史概述.
描述DNA提取和NGS图书馆准备的实验室方法.
通过使用NGS数据,为基因型分析提供用户友好的生物信息管道.

主要方法:

用于Illumina MiSeq测序的DNA提取和图书馆准备.
生物信息学工具用于处理NGS数据的应用.
多部位序列类型 (MLST) 用于基因型确定.

主要成果:

对于P. aeruginosa基因组测序的NGS的成功应用.
开发和描述可访问的生物信息学工作流程.
使用MLST和最小跨度树的基因型推断和可视化.

结论:

NGS技术和生物信息学方法为P. aeruginosa研究提供了强大的工具.
描述的方法有助于基因型分析和进化研究.
本章是研究人员进入P. aeruginosa基因组学的实用指南.

关键词:

提取DNA 提取DNA的方法基因组测序是指基因组的测序.基因型定制是指基因型定制.最少跨越树的树木.这是下一代测序.伪omonas空气菌伪omonas空气菌这就是 eBURST.

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