周围血液淋巴细胞在暴露于具有不同物理性质的X射线后对DNA损伤反应有所不同
Simon Sioen1, Louise D'Hondt1, Fien Van Houte1
1Radiobiology group, Department of Human Structure and Repair, Ghent University, Ghent, Belgium.
International journal of radiation biology
|October 11, 2023
概括
血液细胞中的DNA损伤反应根据X射线能量不同. 低能量的X射线会比高能量的X射线对DNA造成更多的损伤,这凸显了光束质量在辐射研究中的重要性.
科学领域:
- 辐射生物学 辐射生物学
- 医学物理 医学物理
背景情况:
- 放射学使用低X射线能量 (<140keV) 进行成像,而放射治疗使用更高的能量 (MeV) 进行瘤根除.
- 将体外辐射研究推断到临床实践中,需要了解由于能量沉积的差异,不同的X射线能量如何影响生物反应.
研究的目的:
- 为了比较外围血液淋巴细胞 (PBL) 的DNA损伤反应 (DDR),这些淋巴细胞暴露于具有不同光束质量的X射线,平均光子能量 (MPE) 和剂量速率.
- 研究物理参数对细胞对辐射反应的影响.
主要方法:
- 在PBL中使用x-H2AX焦点,微核和SYTOX细胞死亡试验评估DDR.
- 用220kVp的X射线 (SARRP) 和6 MV的X射线 (线性加速器) 辐射细胞.
- 不同的光束质量,MPE (使用Cu过) 和剂量速率 (0.33-6 Gy/min) 来分析它们的影响.
主要成果:
- 220kVp的X射线诱导显著更高的染色体损伤和最初的DNA双链断裂 (DSBs) 与6 MV的X射线相比.
- 通过Cu过增加的MPE显著减少了染色体损伤和DSBs.
- 细胞死亡诱导在能量之间是相似的,特定的抑制剂没有阻止辐射诱导的细胞死亡. 剂量速率的变化在测试范围内没有显示出任何特定影响.
结论:
- 在PBL中,DNA损伤反应受到X射线束质量和平均光子能量的显著影响.
- 这些发现强调了在辐射研究中考虑所有物理参数的必要性,以便准确地将体外结果推断到临床应用中.
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