相关实验视频

Updated: Jul 13, 2025

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation
09:12

DNAzyme-dependent Analysis of rRNA 2’-O-Methylation

Published on: September 16, 2019

8.3K

BERT2OME:基于BERT的变压器架构通过RNA序列的2'-O-甲基化修饰的预测

Necla Nisa Soylu, Emre Sefer

    IEEE/ACM transactions on computational biology and bioinformatics
    |October 11, 2023
    PubMed
    概括

    一种新的方法Bert2Ome使用来自变压器的双向编码器表示 (BERT) 和CNN来有效预测2'-O-甲基化RNA修改位点. 这种方法加速了生物发现,并超越了现有的技术.

    更多相关视频

    Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs08:49

    Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

    Published on: September 16, 2019

    7.7K
    Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis08:50

    Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

    Published on: May 14, 2020

    6.7K

    相关概念视频

    Transfer RNA Synthesis02:36

    Transfer RNA Synthesis

    One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
    Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
    12.0K
    RNA-seq03:21

    RNA-seq

    RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
    Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
    10.0K
    RNA Stability01:53

    RNA Stability

    Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
    33.6K
    RNA Editing02:23

    RNA Editing

    RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
    9.0K
    Improving Translational Accuracy02:07

    Improving Translational Accuracy

    Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
    11.4K
    Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

    Pre-mRNA Processing: Modification of pre-mRNA Ends

    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
    9.4K