来自细菌和古生物的多种受体中的氨基酸识别域是从通用氨基酸传感器进化而来的
Jean Paul Cerna-Vargas1,2, Vadim M Gumerov3, Tino Krell1
1Department of Biotechnology and Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Granada 18008, Spain.
概括
研究人员确定了一种特定的序列图案,用于核细胞中氨酸感应域. 这一发现有助于理解细菌如何检测环境信号,如生物原氨酸,对于它们的适应和功能至关重要.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 细菌利用多种不同的受体进行环境感知和适应.
- 识别激活这些受体的信号分子仍然是微生物学中的一个重大挑战.
- 生物胺是微生物和人类生理学的重要分子,作为生长基质,神经递质和 osmoprotectants.
研究的目的:
- 为了识别与Cache超级家族内的氨酸感应传感器域相关的特定序列动机.
- 调查这些氨基识别域在细菌和古物种中的流行和功能.
- 阐明氨基识别域的进化起源.
主要方法:
- 在 prokaryotic 传感器域中对特定序列基因进行生物信息选.
- 识别了大约13000个含有该动机的传感器蛋白质,遍及8000个物种.
- 使用复合库选和微热度计定位的实验验证.
- 分析突变结合亲缘关系和与氨基酸感应域的进化比较.
主要成果:
- 确定了Cache超级家族中氨酸感应域特有的保存序列动机.
- 这种基因在各种传感器蛋白中被发现,包括histidine激酶和化学受体,在许多细菌和古生物物种中.
- 实验数据证实了生物氨基胺与选定的传感器领域的特定结合.
- 突变分析证明了该基因在氨酸结合亲和力中的关键作用.
- 氨基酸识别域被证明是从通用氨基酸感应的Cache域进化而来的.
结论:
- 鉴定出来的基因为检测 prokaryotic 受体中的氨基酸感应能力提供了可靠的标记.
- 这一发现有助于对许多不具特征的监管系统进行功能性描述.
- 这项研究提供了细菌传感器蛋白中信号识别演变的见解.
- 这种方法可以实现有针对性的实验验证,加速发现新的信号受体相互作用.
更多相关视频
06:50Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
Published on: January 26, 2024
1.9K
11:47Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
Published on: August 1, 2016
16.0K
相关概念视频
01:49Conserved Binding Sites
4.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.2K
02:26tRNA Activation
19.3K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
19.3K
02:26Conservation of Protein Domains Over Different Proteins
10.9K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
10.9K
01:27Diversity of Archaea III
17
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
17
01:21Three-Domain System of Life
33
Ribosomal RNA (rRNA) sequence analysis revealed three distinct groups of cells: eukaryotes, bacteria, and archaea. In 1978, Carl R. Woese proposed the concept of domains, a taxonomic level above kingdoms, to differentiate these groups. He suggested that archaea and bacteria, despite their similar appearance, represent separate domains. Domains differ in rRNA, membrane lipid structure, transfer RNA, and antibiotic sensitivity.In this classification, animals, plants, and fungi belong to the...
33
01:24Diversity of Archaea II
21
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
21