在病毒进入过程中,人类乳头瘤病毒16型L2囊蛋白预测的长乱段的序列独立活性
Changin Oh1, Patrick M Buckley2, Jeongjoon Choi1
1Department of Genetics, Yale School of Medicine, New Haven, CT 06520-8005.
概括
一个病毒蛋白细分体.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 人类乳头瘤病毒 (HPV) 的L2囊蛋白对病毒进入和细胞内贩运至关重要.
- 在进入过程中,L2必须突出进入细胞质,与细胞因子 (如复原体) 相互作用.
- 蛋白质的功能通常与氨基酸序列和组成有关.
研究的目的:
- 为了研究特定的L2蛋白段在HPV16伪病毒感染中的序列,组成和长度的作用.
- 要确定这个段的函数是依赖序列还是独立的.
主要方法:
- 分析L2蛋白的~110个氨基酸失序部分内的大和小的框架内删除.
- 通过插入具有不同序列和组成的多种蛋白质段来恢复感染力.
- 在伪病毒感染模型中评估L2细胞质突起,复原体协会和病毒贩运.
主要成果:
- 在L2段的大缺失取消了HPV16伪病毒的感染性,通过防止细胞质突起和逆转激素结合.
- 通过插入不同序列和组合的段落来恢复传染性,包括混的段落.
- 带有小缺失的突变的传染性与剩余部分的大小直接相关.
结论:
- 对于HPV16伪病毒感染而言,无序L2段的长度,而不是序列或组成,至关重要.
- 对于L2有效地与细胞质贩运机器相互作用,需要最小的长度.
- 这项研究证明了像病毒感染这样的复杂生物过程中的序列独立蛋白质功能,这对蛋白质进化有影响.
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