在Chlorella pyrenoidosa和Rhodobacter sphaeroides的共同培养中评估营养限制
Xiao-Fei Shen1, Ya-Ping Xu1, Yi-Fan Jiang1
1School of Ecology and Environment, Anhui Normal University, Wuhu, Anhui 241000, PR China.
The Science of the total environment
|October 11, 2023
概括
微藻-光合作用细菌共同培养中的缺乏提高了石油生产,但减少了生物质. 足够的是高生长和脂质产量的关键,营养素限制影响脂肪酸和.
科学领域:
- 生物技术是生物技术.
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 微藻-细菌共培被用于生物质和脂质生产.
- 大多数研究都集中在固细菌上,不清楚非固光合作用细菌 (PSB) 在缺乏下的影响.
研究的目的:
- 调查 (N) 饥饿和 (P) 供应对微藻 (Chlorella pyrenoidosa) 和PSB (Rhodobacter sphaeroides) 的共同培养中的石油生产的影响.
- 了解这种光异质共同培养系统中N饥饿对脂质积累的潜在机制.
主要方法:
- 使用乙酸盐在光异质变条件下培养Chlorella pyrenoidosa和Rhodobacter sphaeroides共同培养.
- 操纵的饥饿和供应水平.
- 脂肪酸甲基 (FAME) 含量,产量,生物质和生产率的分析.
- 利用iTRAQ (相对和绝对量化等离子标签) 分析来研究蛋白质组变化.
主要成果:
- 在足够的下,N饥饿显著增加了FAME含量 (21.7%至28.2%) 和产量 (0.17至0.22),但减少了生物质和FAME生产率.
- 足够的对于高增长率和FAME生产率至关重要.
- 无论是N和P的缺陷都减少了不和FAME的比例.
- iTRAQ分析显示,N饥饿会将碳流转向微藻中的脂肪酸合成.
结论:
- 饥饿可以增强微藻-PSB共同培养中的脂质积累,特别是当足够时.
- 在光异质培养中,优化营养条件 (N和P水平) 对于最大限度地提高生物质和脂质生产至关重要.
- 需要进一步的研究来阐明微藻-细菌相互作用的复杂机制,以改善生物燃料生产的共同培养系统.
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