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对人类冠状病毒229E的尖端蛋白和其宿主适应性的Codon使用偏差分析
Meng Lu1, Wenbo Wan1, Yuxing Li1
1Shanghai Institute of Infectious Disease and Biosecurity, School of Public Health, Fudan University, 131 Dong'an Road, Shanghai 200032, People's Republic of China.
International journal of biological macromolecules
|October 11, 2023
概括
人类冠状病毒229E (HCoV-229E) 显示低密码子使用偏差,受自然选择的影响. 它的遗传模式与驼非常相似,这表明它与动物有传染病联系,有助于理解病毒进化.
科学领域:
- 病毒学 病毒学
- 分子进化分子进化
- 基因组学就是基因组学.
背景情况:
- 人类冠状病毒229E (HCoV-229E) 在人类中引起轻度呼吸系统疾病.
- HCoV-229E是一种动物源的动物性病毒.
- 了解病毒遗传特征对于公共卫生至关重要.
研究的目的:
- 进行对HCoV-229E尖峰 (S) 基因进行全面的遗传学和编码子使用分析.
- 调查HCoV-229E.的进化轨迹和宿主关系.
- 为了比较HCoV-229E密码子的使用与潜在宿主物种.
主要方法:
- 遗传学分析以确定病毒聚类和宿主关联.
- 主要成分分析 (PCA) 用于遗传模式可视化.
- 使用Codon适应指数 (CAI) 和相对Codon降低优化指数 (RCDI) 的Codon使用偏差分析.
主要成果:
- 根据遗传学分析,HCoV-229E被分为四个不同的组,每个组都有独特的宿主归属.
- 在S基因中Codon使用偏差很低,主要是由自然选择,突变压力和二核酸丰富度形成的.
- HCoV-229E编码子的使用模式与驼相比,与驼或人类相比更接近.
结论:
- 这项研究阐明了HCoV-229E的编码子使用模式,为其进化历史提供了洞察力.
- 研究结果表明,HCoV-229E与驼之间存在更密切的遗传关系,这进一步证实了它的动物源.
- 这项研究有助于更深入地了解冠状病毒的演变和物种间传播.
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