在成年感官神经元和皮肤再生神经中Protocadherin γ的作用
Rebecca M Long1, Honyi Ong1, Wendy Xueyi Wang2,3
1Division of Neurology, Department of Medicine and the Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta T6G 2G3, Canada.
概括
玛-原cadherins (Pcdhγ) 调节感觉轴突的生长和皮肤中的相互作用. 击败神经元中的Pcdhγ增强了外生长,而皮肤Pcdhγ的减少则加速了皮肤外皮再生和受伤后的分支.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 集群原始干素 (cPcdhs) 对于通过细胞与细胞相互作用在中枢神经系统中塑造神经元树木的模式至关重要.
- 原cadherins在外周感官 afferents 内化表皮和指导空间信号忠实性的作用仍然未被探索.
研究的目的:
- 调查在皮肤再内核化过程中成年感官神经元和表皮皮质角质细胞中马-原蛋白激素 (Pcdhγ) 的表达和功能.
- 确定Pcdhγ调制对感觉轴突外生长,分支和与角质细胞相互作用的影响.
主要方法:
- 利用小干扰RNA (siRNA) 和腺相关病毒 (AAV) -Cre重组酶来击败Pcdhγ在成年小鼠初级感觉神经元中.
- 在培养的神经元中检查了神经元外生长,分支,自我交叉和邻居瓦.
- 在本地后腿皮肤Pcdhγ敲击后,评估了皮肤表皮再生,轴突分支和状细胞特征 in vivo.
主要成果:
- 在感觉神经元中,PCDHAG的敲击显著增加了神经元的增长和分支,这种效应被Rac1抑制所废除.
- 敲击导致神经元自我交叉和邻居瓦的增加,这表明在感觉轴突排斥中发挥了作用.
- 在体内,Pcdhγ在皮肤中的敲击加速了表皮膜再内核,增加了轴突末端的分支,减少了轴突内部的间距,对角质细胞有相应的影响.
结论:
- Pcdhγ在减弱感觉轴突外生长和调节皮肤再内核过程中的轴突-轴突和轴突-角质细胞相互作用方面发挥着关键作用.
- 调节Pcdhγ为增强外围神经损伤后的再生结果提供了潜在的治疗策略.
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