一个依赖于时钟的车,用于在背中介质下丘脑中节律性唤醒
Qiang Liu1, Benjamin J Bell1,2, Dong Won Kim3,4,5
1Department of Neurology, Johns Hopkins University, Baltimore, MD, 21205, USA.
Nature communications
|October 11, 2023
概括
昼夜时钟使用mWAKE分子来控制兴奋. 这种分子在夜间的兴奋中起到制动作用,确保小鼠的正常睡眠和清醒周期.
科学领域:
- 神经科学是一个神经科学.
- 时间生物学 时间生物学
- 分子生物学分子生物学
背景情况:
- 循环时钟调节兴奋节奏,但分子机制尚未完全理解.
- 果中的Wide Awake (WAKE) 蛋白质影响睡眠和唤醒.
- 哺乳动物中WAKE同类的功能在很大程度上仍未被探索.
研究的目的:
- 研究小鼠WAKE同类 (mWAKE/ANKFN1) 在调节兴奋中的作用.
- 为了确定神经回路和分子机制,基础的昼夜兴奋控制.
- 为了阐明昼夜时钟如何调节哺乳动物的兴奋水平.
主要方法:
- 在小鼠的背中中垂体 (DMH) 神经元的体内成像.
- 补丁电生理学记录神经元发射模式.
- 化学遗传学和光遗传学来操纵DMHmWAKE神经元活动.
- 单细胞RNA测序以对DMHmWAKE神经元进行分析.
主要成果:
- mWAKE标记了DMH神经元的一个亚群,这些神经元对于兴奋至关重要.
- DMHmWAKE神经元活动在清醒和REM睡眠期间升高,特别是在晚上.
- 对于促进兴奋,GABAergic DMHmWAKE 神经元是必要的,也是足够的.
- mWAKE作为昼夜制动器,在小鼠通常活跃的夜间减少激发.
结论:
- 生物钟采用相反的机制,包括mWAKE介导的抑制,以微调唤醒.
- mWAKE在关闭时间依赖性兴奋方面发挥着至关重要的作用.
- 干扰mWAKE导致过度兴奋,突出其在维持睡眠-清醒平衡中的重要性.
相关概念视频
Sleep-Wake Cycles
1.4K
Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
1.4K
Functional Brain Systems: Reticular Formation
2.0K
The reticular formation is a complex network of gray and white matter located within the brainstem extending from the medulla to the midbrain.
Within the reticular formation, there are several distinct nuclei that can be classified into three broad categories. The Raphe nuclei are located along the midline of the brainstem. They are primarily known for their role in synthesizing and releasing serotonin, a neurotransmitter involved in regulating mood, appetite, sleep, and circadian rhythms. The...
Within the reticular formation, there are several distinct nuclei that can be classified into three broad categories. The Raphe nuclei are located along the midline of the brainstem. They are primarily known for their role in synthesizing and releasing serotonin, a neurotransmitter involved in regulating mood, appetite, sleep, and circadian rhythms. The...
2.0K
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Diencephalon: Hypothalamus and Coordination
1.6K
The hypothalamus is a small yet highly complex and essential brain region that plays a crucial role in regulating various bodily functions. Anatomically, it is located at the base of the brain, just above the brainstem and below the thalamus, forming part of the limbic system.
The hypothalamus interacts with other brain regions, including the pituitary gland, through a direct physical connection called the hypothalamic-pituitary axis. The hypothalamus receives somatic and visceral inputs and...
The hypothalamus interacts with other brain regions, including the pituitary gland, through a direct physical connection called the hypothalamic-pituitary axis. The hypothalamus receives somatic and visceral inputs and...
1.6K
REM Sleep Behavior Disorder
213
REM Sleep Behavior Disorder (RBD) is a sleep disorder characterized by the absence of muscle paralysis that normally occurs during the REM phase of sleep. This absence allows individuals to physically act out their dreams, which are often vivid and disturbing. Common behaviors exhibited during episodes include kicking, punching, and yelling. These actions can be dangerous, potentially leading to injuries for the person with RBD or their bed partner.
RBD is significantly associated with...
RBD is significantly associated with...
213
Optimal Arousal Theory
202
The optimal arousal theory suggests that performance is maximized when an individual experiences a moderate level of arousal. This theory is closely tied to the Yerkes-Dodson law, which illustrates an inverted U-shaped relationship between arousal and performance. The law, formulated by psychologists Robert Yerkes and John Dodson, implies an ideal arousal level for optimal performance, and deviations from this level can lead to declines in effectiveness.
Inverted U-Shaped Performance Curve
The...
Inverted U-Shaped Performance Curve
The...
202


