用条件生成模型揭示看不见的细胞表型
Alexis Lamiable1, Tiphaine Champetier1,2, Francesco Leonardi1,3
1Computational Bioimaging and Bioinformatics, Institut de Biologie de l'Ecole Normale Supérieure, PSL University, 46, rue d'Ulm, 75005, Paris, France.
Nature communications
|October 11, 2023
概括
这项研究引入了使用条件生成模型来取消自然细胞变异性的合成细胞扰动. 这种方法揭示了微妙的,否则看不见的细胞表型,对于医学和生物学中生物标志物发现至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 计算生物学是一种计算生物学.
- 生物医学成像学 生物医学成像学
背景情况:
- 细胞表型扰动和显微镜观察在生物科学,药物发现和医学中至关重要.
- 自然细胞的变异性往往掩盖了微妙的表型变化,阻碍了准确的观察和分析.
研究的目的:
- 开发一种在显微镜图像中取消细胞变异的方法.
- 为了能够检测微妙的或其他看不见的细胞表型.
- 促进生物和疾病生物标志物的发现.
主要方法:
- 使用条件生成模型在不同条件之间转换细胞图像.
- 应用合成细胞扰动来取消固有的细胞变性.
- 合成扰动原理的视觉和定量验证.
主要成果:
- 证明了条件生成模型在转换细胞图像中的有效性.
- 成功取消了自然细胞的变异性,允许更清晰的表型观察.
- 在寄生虫感染,神经元疾病突变和低剂量药物治疗的情况下,揭示了以前隐藏的细胞表型.
结论:
- 条件生成模型为合成细胞扰动提供了强大的和可访问的方法.
- 该方法增强了微妙的生物和疾病生物标志物的发现.
- 这种技术对促进细胞生物学,药物发现和医学诊断具有广泛的影响.
相关概念视频
02:32Position-effect Variegation
6.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
6.3K
01:58Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
02:11Reporter Genes
11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
02:15Replicative Cell Senescence
3.7K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.7K
01:22iPS Cell Differentiation
2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K
00:57Cellular Differentiation
2.7K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
A zygote is a...
2.7K