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系统性炎症和肠道微生物群对年轻和中年老鼠骨折的反应

Joseph L Roberts^1,2,3, Brandon Chiedo⁴, Hicham Drissi^5,6

¹Department of Orthopaedics, Emory University School of Medicine, 21 Ortho Ln, 6th Fl, Office 12, Atlanta, GA, 30329, USA. joseph.roberts.3@asu.edu.

|October 11, 2023

概括

衰老通过增加炎症和改变肠道微生物群来加剧骨折愈合. 中年老鼠在骨骨折后的年轻小鼠相比,显示出更高的促炎细胞因子和破坏肠道细菌.

科学领域:

整形外科整形外科整形外科
免疫学免疫学免疫学
微生物组研究微生物组研究

背景情况:

年龄显著影响骨折愈合和恢复.
增强的炎症是老年患者中延迟愈合的一个可疑因素.
系统和肠道微生物对衰老中骨折的反应尚未完全理解.

研究的目的:

为了研究系统性细胞因子和肠道微生物组对年轻成年人与中年老鼠的股骨骨折的反应.
为了确定炎症标志物和骨折后肠道微生物组成的年龄相关差异.

主要方法:

在年轻 (3个月大) 和中年 (15个月大) 雌性野生型小鼠中诱导了关闭的股骨骨折.
血清细胞因子水平在骨折后的第10,14和18天被测量.
用测序分析了肠道微生物群的组成.

主要成果:

中年老鼠表现出明显的血清细胞因子概况,其中IL-17a,IL-10,IL-6,MCP-1,EPO和TNFα的水平升高.
在骨折后的第三天,肠道微生物群结构在两个年龄组都发生了变化.
中年老鼠表现出肠杆菌群的开花和增加的Muribaculum,而年轻的小鼠则增加了Bifidobacterium.
在Bifidobacterium和IL-17a.之间发现了负相关性.

结论:

衰老加剧了对骨折的炎症反应.
骨折导致肠道微生物群失调,在中年人群中更为明显.
细胞因子和肠道微生物群的与年龄相关的变化可能导致骨折愈合的延迟.

关键词:

衰老的衰老衰老的衰老它们的IL-17a是IL-17a.炎症炎症是一种炎症.微生物群中的微生物群二次性骨愈合的方法

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