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使用光学连贯性断层扫描在体内表征田间茎壁厚度

Qi Fang^1,2, Felipe A Castro-Urrea^3,4, Felix Haederle⁵

¹Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Crawley, WA, 6009, Australia. qi.fang@uwa.edu.au.

|October 11, 2023

概括

光学连贯断层扫描 (OCT) 提供了一种快速的,非破坏性的方法来测量现生植物中的野豆茎壁厚度. 这种技术准确地评估了干部特征,这些特征对于培育耐住宿品种至关重要.

科学领域:

植物育种植物育种
农业工程农业工程
生物光子学生物光子学

背景情况:

田间豆育种需要选择强壮的茎,以防止沉积.
传统的树干强度评估方法具有破坏性,可能不反映活植物的强度.
光学连贯断层扫描 (OCT) 为体内干部分析提供了一种非接触,非破坏性的方法.

研究的目的:

为了证明OCT在野外豆的体内茎壁厚度测量中的有效性.
为了更准确地比较体内OCT测量与体外数据.
为了研究不同田间豆品种沿内部节的茎壁厚度和宽度变化.

主要方法:

实时,在野豆茎的体内成像使用OCT.
对OCT对干组织折射率的测量进行校正.
在体内OCT测量的比较与切割茎的体外测量.
分析多个内部节点位置的茎壁厚度和宽度.

主要成果:

实体内OCT对茎壁厚度的测量显示,与实体外结果相比,平均百分比误差为-3.1%.
种植者Dunwa表现出统一的茎壁厚度,而Kaspa显示在内部节点的长度下降.
两种品种都在内部节点上增加了茎幅,但Dunwa的速度是Kaspa的三倍.

结论:

OCT是一种有效的工具,可以准确地在体内测量田间豆茎壁厚度.
在Dunwa和Kaspa之间观察到茎壁厚度和内部节点的宽度的明显模式,这表明茎强度的潜在差异.
这种快速的OCT成像方法有助于描述育种耐住宿的田间豆品种的物理特征.

关键词:

田间豆是一种田间豆.在体内测量体内测量.光学连贯性断层扫描仪树干结构树干结构茎壁的厚度茎壁的厚度

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