加快表观遗传年龄,炎症和肺部基因表达:吸烟者和吸烟者与非吸烟者的比较
Min-Ae Song1, Kellie M Mori2, Joseph P McElroy3
1Division of Environmental Health Sciences, College of Public Health, The Ohio State University, 404 Cunz Hall, 1841 Neil Ave., Columbus, OH, 43210, USA. Song.991@osu.edu.
Clinical epigenetics
|October 11, 2023
概括
电子烟蒸汽和吸烟加速肺部表观遗传衰老,影响肺部健康. 这项研究揭示了与非使用者相比,蒸汽和吸烟者对生物衰老标志物的不良影响.
科学领域:
- 肺部医学 肺部医学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 毒理学 毒理学 毒理学
背景情况:
- 吸烟和衰老是肺部疾病的主要危险因素.
- 表观遗传衰老可能与吸烟,电子烟和肺部健康有关.
- 没有先前的研究探讨吸烟/vaping相关的表观遗传衰老与肺部生物标志物.
研究的目的:
- 评估年轻电子烟 (EC) 用户,吸烟者 (SM) 和非吸烟者/非吸烟者 (NS) 的肺部表观遗传衰老 (DNA甲基化).
- 调查表观遗传衰老措施与时间学年龄,寿命,死亡率,端粒长度,吸烟/吸烟史和肺部生物标志物之间的关联.
- 确定表观遗传衰老与肺细胞因子和基因表达之间的关系.
主要方法:
- 在EC (n=14),SM (n=16) 和NS (n=39) 中使用DNA甲基化评估肺部表观遗传衰老 (mAge) 和加速 (mAA),年龄为21-30岁.
- 检查的mAge估计与时间学年龄 (Horvath-mAge),寿命/死亡率 (Grim-mAge) 和端粒长度 (TL-mAge) 相比.
- 分析了与EC/吸烟史,尿路生物标志物,肺细胞因子 (IL-1β,IL-6,IL-8) 和转录组的关联.
主要成果:
- 与NS相比,EC和SM的Grim-mAge显著更老,TL-mAge更短.
- 在EC和SM中,Grim-mAge显著加速,而TL-mAge则减缓.
- 在SM中,Grim-mAA与尼古丁摄入量和NNAL相关;EC的Horvath-mAA与吸气频率相关. 格林-mAA相关基因与免疫路径和细胞结构有关.
结论:
- 吸烟者的加速肺部表观遗传衰老与之前的血液研究一致.
- 电子烟使用者肺部衰老的加快表明,电子烟可能会对肺部产生不良影响.
- 这些发现凸显了对表观遗传标记和吸烟和蒸汽烟对肺部的影响,特别是ECs独特影响的进一步研究的需要.
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