AMP 脱氨酶在花环化物中的作用:对利用和脂质生物合成的影响
Shaoqi Li1, Ghizlane Tahiri2, Junhuan Yang3
1Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo 255000, China.
Journal of agricultural and food chemistry
|October 12, 2023
概括
AMP脱氨酶 (AMPD) 调节了Mucor circinelloide中的脂质积累. 删除AMPD基因增加了脂质含量,而过度表达它们在富含的条件下促进了生长和脂质的产生.
科学领域:
- 生物化学 生物化学
- 菌类学 菌类学是指菌类学.
- 代谢工程是代谢工程.
背景情况:
- 油性生物体中的脂质积累通常是由耗引起的,涉及腺单酸盐 (AMP) 调节.
- 在油性真菌Mucor circinelloide的脂质生成中AMP脱氨酶 (AMPD) 的特定作用仍然在很大程度上未被探索.
研究的目的:
- 在M. circinelloide中识别AMPD编码基因.
- 通过基因操纵 (过度表达和删除) 阐明AMPD在真菌脂质合成中的功能.
主要方法:
- 在M. circinelloide中识别和表征AMPD基因 (ampd1和ampd2).
- 基因删除和过度表达策略研究AMPD功能.
- 测量脂质含量,酶活性,分析代谢和能量平衡.
主要成果:
- 在有限的条件下,AMPD1和AMPD2基因的删除导致脂质含量显著增加 (21%和28%).
- 过度表达AMPD基因增强了细胞生长和脂质积累,即使在富含的条件下.
- AMPD操纵改变了消耗,能量平衡和脂质酶的活性,模仿了限制反应.
结论:
- 在M. circinelloide中,AMPD在调节和能量代谢方面起着至关重要的作用.
- 过度表达AMPD基因可以通过模拟限,在富含的条件下诱导显著的脂质积累.
- 这项研究揭示了AMPD在控制M.circinelloide的脂质发生过程中的新功能.
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