呼吸道上皮细胞对菌根菌感染产生早期反应
Amy M Barclay1, Dennis K Ninaber2, Suzanne van Veen1
1Department of Infectious Diseases, Leiden University Medical Center, Leiden, Netherlands.
Frontiers in cellular and infection microbiology
|October 12, 2023
概括
空气道上皮细胞被各种真菌菌差异性感染. 这些细胞迅速升高抗微生物的调节,并分泌炎症信号,吸引中性粒细胞控制感染.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 微生物学 微生物学
背景情况:
- 肺上皮细胞是对吸入病原体的主要防御.
- 了解表皮对菌根细菌的反应对于解释感染结果至关重要.
- 呼吸道上皮在真菌细菌感染中的作用需要进一步描述.
研究的目的:
- 研究人类原发性支气管上皮细胞 (ALI-PBEC) 对感染Mycobacterium tuberculosis (Mtb),M. bovis (BCG),M. avium和M. smegmatis的反应.
- 为了分析不同基因表达和分泌因子在肌肉细菌感染后分泌的因素.
- 评估表皮分泌物在吸引中性粒细胞中的作用.
主要方法:
- 使用一种空气-液体接口模型与人类初级支气管上皮细胞 (ALI-PBEC).
- 感染了四种不同的真菌细菌物种的ALI-PBEC.
- 进行了差异基因表达分析,并分析了角和基底分泌物.
- 评估中性粒细胞对上皮质分泌物的吸引力.
主要成果:
- 这四种真菌细菌都感染了ALI-PBEC,感染频率各不相同 (Mtb的感染频率最低,M. avium的感染频率最高).
- 常见的基因表达变化包括BIRC3,S100A8,DEFB4的上调和BPIFB1.1的下调.
- 角分泌物含有丰富的促炎细胞因子,而基底分泌物含有生长因子和化学因子.
- 中性粒细胞被大多数感染细胞的分泌物所吸引,除了那些感染M. avium的细胞.
结论:
- 空气道上皮细胞对真菌细菌感染具有差异性的敏感性.
- 皮质细胞具有快速反应,涉及抗微生物基因上调和中性粒细胞吸引因子的分泌.
- 呼吸道上皮在控制和调节菌根菌感染方面发挥着重要作用.
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