控制由原生和甲基化狼11S球蛋白形成的细菌生物膜
Gamal Enan1, Seham Abdel-Shafi1, Mona El-Nemr1
1Department of Botany and Microbiology, Faculty of Science, Zagazig University, Zagazig, Egypt.
Frontiers in microbiology
|October 12, 2023
概括
狼种子球蛋白 (11S) 和它们的甲基化形式 (M11S) 显示出强大的抗菌和抗生物膜活性,在对抗病原性细菌方面表现优于一些抗生素. M11S显示MIC和MBIC值明显较低,有效抑制细菌生长和生物膜形成.
科学领域:
- 农业科学 农业科学
- 生物化学 生物化学
- 微生物学 微生物学
背景情况:
- 植物衍生蛋白质正在探索其新型抗微生物特性.
- 狼11S球蛋白及其衍生物具有潜在的治疗应用.
- 抗生素耐药性需要寻找替代抗菌剂.
研究的目的:
- 评估狼11S球蛋白及其甲基化衍生物 (M11S) 的抗菌和抗生物膜功效.
- 将11S和M11S对病原细菌的活性与现有的抗生素进行比较.
- 阐明对抗细菌生长和生物膜形成的作用机制.
主要方法:
- 最低抑制度 (MIC) 对七种致病细菌的测定.
- 最低生物膜抑制度 (MBIC) 的确定.
- 扫描电子显微镜 (SEM) 用于生物膜结构分析.
主要成果:
- 与11S相比,M11S的MIC显著低于11S,以及一些抗生素对阳性和阴性细菌的抗生素.
- 11S和M11S都有效地减少了液体细菌的生长,并抑制了生物膜的形成.
- 与11S相比,M11S显示出更高的抗生物膜活性,在MBIC的玻璃幻灯片上完全预防了生物膜.
- SEM揭示了生物膜变形,表明细胞膜向机制.
结论:
- 狼11S球蛋白和M11S具有强大的抗菌和抗生物膜特性.
- M11S是一种高效的抗微生物剂,性能优于11S和某些抗生素.
- 这些化合物显示出作为天然替代品对抗细菌感染和生物膜形成的承诺.
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