通过烯合成酶解码催化
Joshua N Whitehead1, Nicole G H Leferink2, Linus O Johannissen1
1Manchester Institute of Biotechnology, Department of Chemistry, The University of Manchester, Manchester, M1 7DN, United Kingdom.
ACS catalysis
|October 12, 2023
概括
本综述更新了合成酶/循环酶 (TS) 生物催化学的领域,详细介绍了了解TS反应化学和工程方面的最新进展. 未来的目标包括合理设计"设计合成体"用于新型应用.
科学领域:
- 生物化学和合成生物学
- 酶催化和工程 酶催化和工程
背景情况:
- 合成酶 (TSs) 是生产各种类的关键酶.
- 2017年的一项审查总结了基础的TS发现;这项工作更新了自那时以来的领域.
研究的目的:
- 审查最近的TS文献,重点关注反应化学和工程策略.
- 探索合成酶的合理设计的未来.
主要方法:
- 2017年以后发表的TS研究的文献综述.
- 综合实验和计算方法的分析,以了解TS催化.
- 检查最近的TS工程策略,包括机器学习.
主要成果:
- 确定控制TS产品结果的催化动机.
- 详细探讨TS酶如何将简单的基质转化为复杂的 terpenoids.
- 新兴的TS工程数据驱动方法.
结论:
- 对TS的理性和预测性工程正在成为一个现实的目标.
- 了解催化动机是解读TS复杂性的关键.
- "设计者合成酶"已经在地平线上.
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