通过循环应变增强活力的作用力在绵羊的动脉动脉光滑肌肉中

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¹Centre for Heart Lung Innovation, St. Paul's Hospital/Providence Health Care, University of British Columbia, Vancouver, British Columbia, Canada.

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概括

循环菌株通过蛋白激酶C (PKC) 和rho-激酶 (ROCK) 信号,在大动脉中增强力. 集成链激酶 (ILK) 在上游作用,调解这种机械应变诱导的力强化.

科学领域:

血管生理学血管生理学
分子生物学分子生物学
生物化学生物化学

背景情况:

大动脉因血压波动而经历周期性应变.
这种机械应变增加了动脉光滑肌肉的力量产生,这种现象被称为"力量强化".
这种强化不同于较小动脉中的肌源性反应.

研究的目的:

研究蛋白质激酶C (PKC),罗激酶 (ROCK) 和整合因子结合激酶 (ILK) 在绵羊动脉中强力增强中的作用.
阐明机械应变诱导的力强化所涉及的信号通路.

主要方法:

在受循环压力的绵羊动脉中研究了力强化.
利用激酶抑制剂 (PKC,ROCK,ILK) 来评估它们对力强化和髓轻链酸化的影响.
研究了这些激酶在机械传导中的作用.

主要成果:

循环菌株增强了激活肌肉中的调节性髓光链酸化.
ROCK 抑制降低了力强化和活性力,这表明肌酸氨酸轻链酸酶消抑制.
通过PKC抑制,消除了强力增强,这表明PKC在这种现象中发挥了特殊作用.
ILK抑制模仿了PKC抑制,这表明ILK在这个途径上是PKC的上游.

结论:

ILK,PKC和ROCK是信号传导通路中的关键激酶,它们通过大动脉中的机械应变来调解大动脉中的力强化.
ILK可能会在PKC的上游作用,调解机械刺激的影响.
这些发现有助于了解大动脉中血压的自调节.

关键词:

整合素连接激酶的整合因子肌肉机械学肌肉机械学肌酸氨酸光链化酸化蛋白质激酶 C 的蛋白质激酶 C.罗基纳酶 (rho-kinase) 是一种

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