基于黄金纳米颗粒的免疫染色学测定用于快速检测伊马萨利尔
Xinxin Xu1,2, Aihong Wu1,2, Lingling Guo1,2
1International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China. murel@163.com.
Nanoscale horizons
|October 12, 2023
概括
这项研究开发了一种快速免疫染色学试验,用于检测农产品中的杀菌剂imazalil (IMZ). 该测定可提供IMZ残留的敏感和特定检测,确保食品安全.
科学领域:
- 农业化学 农业化学
- 免疫技术是一种免疫技术.
- 食品安全分析 食品安全分析
背景情况:
- 伊马扎利尔 (IMZ) 是一种广泛使用的农业真菌杀菌剂.
- 农作物中的残留IMZ对人类健康构成潜在的风险.
- 敏感的IMZ检测方法对于食品安全至关重要.
研究的目的:
- 开发一种针对IMZ的敏感单克隆抗体 (mAb).
- 为IMZ检测创建基于黄金纳米粒子的快速免疫染色学试验 (GICA).
- 为了验证GICA在水果中的IMZ现场定量.
主要方法:
- 在小鼠中设计和免疫IMZ哈普以产生抗IMZ mAb (IgG2a亚型).
- 使用ic-ELISA进行mAb灵敏度 (IC50 = 0.95 ng mL-1) 和特异性的表征.
- 在葡萄和西红中检测IMZ的基于金纳米粒子的免疫染色学试验 (GICA) 的开发和应用.
主要成果:
- 抗IMZ mAb具有高灵敏度,与其他化学物质没有交叉反应.
- 该GICA显示,视觉检测极限 (vLOD) 为25 ng g-1和切断值为500 ng g-1.
- 计算的LOD在葡萄中低至4.12 ng g-1,在番茄中低至4.70 ng g-1,恢复率很好 (84.7%-104.4%),变化系数低 (5.7%-11.8%).
结论:
- 开发的GICA提供了一种快速 (30分钟),灵敏和特定的方法,用于现场IMZ检测.
- 该试验对于在葡萄和番茄样本中量化IMZ残留物是实用的.
- 这项技术通过监测杀菌剂污染,有助于确保食品安全.
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