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Updated: Jul 13, 2025

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique
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Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

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BRCA1-BARD1结合了多个染色体识别模块,以弥合新生的核细胞组

Hayden Burdett1, Martina Foglizzo2, Laura J Musgrove2

  • 1Wellcome Centre for Cell Biology, University of Edinburgh, Michael Swann Building, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, UK.

Nucleic acids research
|October 12, 2023
PubMed
概括

该BRCA1-BARD1复合物优先结合二核酶体,桥接它们以增强DNA修复. 这种相互作用机制有助于BRCA1-BARD1克服抑制性基因素标记,促进其E3泛基因酶活性用于同源重组修复.

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Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
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DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
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