通过猪血清抗体中和E型肝炎病毒
Nele Gremmel1, Oliver Keuling2, Martin Eiden3
1Department of Infectious Diseases, Institute of Virology, University of Veterinary Medicine , Hannover, Germany.
Journal of clinical microbiology
|October 12, 2023
概括
生肉消费有感染E型肝炎病毒 (HEV) 的风险. 一种新的基于细胞的测定方法有效地测量了野猪和猪中的中和抗体,有助于HEV疫苗的开发.
科学领域:
- 兽医病毒学 兽医病毒学
- 免疫学 免疫学 免疫学
- 食品安全 食品安全
背景情况:
- 生或未煮熟的肉类消费是人类肝炎E病毒 (HEV) 感染的重要危险因素.
- 在高收入国家,家养猪和野猪是HEV的主要动物库.
- 目前的HEV检测方法包括逆转录PCR和抗体酶相关免疫吸收试验 (ELISA).
研究的目的:
- 建立基于细胞培养的血清中和试验,用于表征对HEV的幽默免疫反应.
- 评估野猪和家猪血清样本中抗体的中和活性.
- 评估开发的试验对评估HEV候选疫苗的有用性.
主要方法:
- 基于细胞培养的血清中和试验是使用培养适应的HEV菌株开发的.
- 通过洗剂处理去除病毒准包裹是抗体测量所必需的.
- 从343只野猪和2只经过实验感染的家猪的血清样本使用ELISA和中和试验进行了分析.
主要成果:
- 19%的野猪血清样本通过ELISA检测出抗HEVIgG的阳性.
- 中和试验结果与ELISA发现有很好的相关性.
- 猪血清抗体中和了人类和猪HEV菌株;中和活性存在于具有HEV抗体和RNA的样本中.
- 经过实验感染的猪在感染后迅速增加中和活性.
结论:
- 开发的基于细胞培养的中和试验对表征HEV特异性幽默性免疫反应有效.
- 该试验可以中和各种HEV菌株,包括人类和猪的分离物.
- 这种测试是评估HEV候选疫苗的疗效和了解HEV传播动态的宝贵工具.
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