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Updated: Jul 13, 2025

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单分子FRET解析蛋白质动力学 - - 从等离子体到数据,六个步骤

Benjamin Vermeer1, Jannick van Ossenbruggen1, Sonja Schmid2

  • 1Laboratory of Biophysics, Wageningen University & Research, Wageningen, The Netherlands.

Methods in molecular biology (Clifton, N.J.)
|October 12, 2023
PubMed
概括

本研究提出了使用单分子弗斯特共振能量转移 (smFRET) 来分析蛋白质动态的综合性协议. 该方法使用热冲击蛋白Hsp90进行了证明,可以详细研究蛋白质构造变化.

关键词:
生物结合的生物结合伴奏者蛋白质 Hsp90 是一种蛋白质动力学 蛋白质动力学蛋白质净化 蛋白质的净化单分子FRETET是一种单分子FRET.表面功能化的功能化.

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