Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Circulating cell type senescence signatures track distinct dimensions of health status and trajectories in human longitudinal cohorts.

Cell reports·2026
Same author

SenCat: Cataloging human cell senescence through multi-omic profiling of multiple senescent primary cell types.

Molecular cell·2026
Same author

SenCat: Cataloging human cell senescence through multiomic profiling of multiple senescent primary cell types.

bioRxiv : the preprint server for biology·2026
Same author

Circulating Cell Type Senescence Signatures Reveal High-Resolution Health Status and Trajectories in Human Longitudinal Studies.

medRxiv : the preprint server for health sciences·2026
Same author

Transcript-guided targeted cell enrichment for scalable single-nucleus RNA sequencing.

Cell genomics·2025
Same author

Senescence: An Overlooked VSMC Phenotype and Therapeutic Opportunity?

Arteriosclerosis, thrombosis, and vascular biology·2025
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
查看所有相关文章

相关实验视频

Updated: Jul 13, 2025

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
08:16

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

995

差异性多分子 (A) 使用纳米孔测序进行尾巴长度分析.

Showkat A Dar1, Sulochan Malla1, Cedric Belair1

  • 1Laboratory of Genetics and Genomics, National Institute on Aging, Intramural Research Program, National Institutes of Health, Baltimore, MD, USA.

Methods in molecular biology (Clifton, N.J.)
|October 12, 2023
PubMed
概括
此摘要是机器生成的。

我们介绍了一种使用纳米孔测序来测量多尾长度变化的新方法. 这种技术有助于通过分析信使RNA稳定性和翻译来理解基因表达调节.

关键词:
死亡化 死亡化不同分析差异分析.纳米电池是一个纳米电池.纳米孔直接RNA测序波利 (Poly) 尾巴 (A) 是一个多重的尾巴.

更多相关视频

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.8K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.6K

相关实验视频

Last Updated: Jul 13, 2025

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques
08:16

Author Spotlight: Exploring the Frontier of mRNA Research with Poly A Tail Analysis Techniques

Published on: January 12, 2024

995
Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.8K
Nanopore DNA Sequencing for Metagenomic Soil Analysis
07:33

Nanopore DNA Sequencing for Metagenomic Soil Analysis

Published on: December 14, 2017

30.6K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.

背景情况:

  • 聚A尾,在RNA的3'端的腺核酸序列,对于转录后基因调节至关重要.
  • 多A尾长动态影响信使RNA的稳定性,翻译效率和衰变速率.
  • 精确测量多种类型的尾巴长度对于理解各种细胞环境中的基因表达至关重要.

研究的目的:

  • 引入一种新的方法来分析多样性 (A) 尾巴长度变化.
  • 为了能够精确量化在不同的实验条件下对多尾长度的变化.
  • 为了更深入地了解多 (A) 尾在基因表达中的调节作用.

主要方法:

  • 利用纳米孔测序技术进行直接的RNA分析.
  • 应用线性混合模型进行统计分析的poly(A) 尾巴长度数据.
  • 开发了一种工作流程,用于测量和比较不同条件下的多尾长度.

主要成果:

  • 通过纳米孔测序成功展示了一种使用纳米孔测序精确测量多尾长度的方法.
  • 展示了在条件之间检测和分析多尾长度差异的能力.
  • 提供了一种强大的方法来研究多种尾动力学.

结论:

  • 开发的方法提供了一个强大的工具,用于研究poly (A) 尾部调节.
  • 纳米孔测序与线性混合模型相结合,提供了一种敏感的方法来分析基因表达动态.
  • 这种技术可以推进对多种尾长度变化的功能意义的研究.