来自墨西哥的Ganoderma tuberculosum (Agaricomycetes) 隔离的化合物的抗寄生虫活性
Victoria Espinosa-García1, Jose J Fernandez2, Desirée San Nicolás-Hernández3
1Instituto de Biotecnología y Ecología Aplicada, Universidad Veracruzana, Av. de las Culturas Veracruzanas 101, 91090 Xalapa, Veracruz, México.
International journal of medicinal mushrooms
|October 12, 2023
概括
这项研究分离了墨西哥Ganoderma tuberculosum的五种化合物,其中两种具有显著的抗寄生虫活性,对Leishmania,Trypanosoma和Acanthamoeba具有显著的抗寄生虫活性,验证了传统医学用途.
科学领域:
- 药用化学 医学化学
- 寄生虫学的寄生虫学
- 菌类学 菌类学是指菌类学.
背景情况:
- 甘菌属在传统亚洲医学中有着悠久的药用历史.
- 墨西哥土著社区使用Ganoderma tuberculosum治疗寄生虫感染,但缺乏化学验证.
- 关于墨西哥G. tuberculosum菌株的抗寄生素特性存在有限的研究.
研究的目的:
- 从墨西哥G. tuberculosum菌株中分离和识别具有抗寄生虫活性的化合物.
- 评估隔离化合物对关键原生虫寄生虫的体外抗寄生素疗效.
- 评估这些化合物对哺乳动物细胞的细胞毒性.
主要方法:
- 从G. tuberculosum (GVL-21) 的果实体中提取的黑提取物.
- 指导分化以分离纯化合物.
- 在体外对抗寄生虫的测定 (IC50) 针对莱什曼亚马逊菌,Trypanosoma cruzi,阿坎萨摩巴 castellanii Neff,和纳格莱里亚 fowleri.
- 细胞毒性测定 (CC50) 对小鼠巨细胞.
主要成果:
- 他们分离了五种化合物:埃尔戈斯特醇 (1),埃尔戈斯塔-4,6,8(14),22-四-3-one (2),埃尔戈斯塔-7,22-迪恩-3β-ol (3),3,5-二-埃尔戈斯塔-7,22-迪恩-6-one (4) 和甘酸DM (5).
- 化合物2和5在体外表现出强大的抗寄生虫活性 (IC50:12.3854.34μM) 对所有测试的寄生虫.
- 隔离的化合物对小鼠巨细胞的细胞毒性较低.
结论:
- 这项研究是首次报告1-5化合物对L. amazonensis,T. cruzi,A. castellanii Neff和N. fowleri的体外抗寄生素活性.
- 这些发现支持Ganoderma物种的传统药用.
- 化合物2和5代表了开发新抗寄生虫剂的有希望的线索.
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