由Pseudomonas citronellolis使用酸作为唯一的碳来源进行mcl-PHA的试点规模生产
Milos Kacanski1, Franz Stelzer2, Meg Walsh3
1University of Natural Resources and Life Sciences, Vienna, Department of Agrobiotechnology, Institute of Environmental Biotechnology, Tulln, Austria.
New biotechnology
|October 12, 2023
概括
这项研究表明,从挥发性脂肪酸中大规模生产中长链多基酸盐 (mcl-PHA). 这种生物基材料由于其类似的特性,显示出可持续应用的潜力.
科学领域:
- 生物技术是生物技术.
- 聚合物科学 聚合物科学
- 可持续材料 可持续材料
背景情况:
- 中长链聚酸 (mcl-PHA) 是一种生物基聚合物,有可能用于环保应用.
- 高昂的生产成本和有限的数据阻碍了mcl-PHA的广泛使用.
- 挥发性脂肪酸 (VFA) 为生物炼油厂提供了低成本,可持续的原料.
研究的目的:
- 调查从VFA中大规模生产mcl-PHA.
- 描述从可持续碳来源生产的mcl-PHA的特性.
- 评估使用Pseudomonas citronellolis用于基于VFA的mcl-PHA合成的可行性.
主要方法:
- 使用Pseudomonas citronellolis (DSMZ 50332) 作为生产菌株.
- 在限条件下采用酸作为主要碳源的料批发发酵工艺.
- 建立了pH-stat养方案,以控制酸添加.
- 通过使用各种分析技术提取并表征得到的mcl-PHA.
主要成果:
- 从两个500升尺度发酵中成功生产了1.76公斤mcl-PHA.
- 该聚合物是3-基酸 (60.7%),3-基酸 (37.3%) 和3-基酸 (2.0%) 的共聚物.
- mcl-PHA具有高重平均分子量 (Mw) 536 kDa,并且含有不和的侧链.
结论:
- 证实了从废物中获得的VFA中大量生产mcl-PHA的潜力.
- 生产的mcl-PHA具有可取的状特性,适合各种应用.
- 不和侧链的存在表明了进一步的聚合物修饰的机会.
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