亚替代剂对PM2.5结合的多环芳香化合物对动脉样斑块的发生和发展产生了负面影响
Yu Zhang1, JingBo Zhao2, Qian Hu1
1Tianjin Key Laboratory of Urban Transport Emission Research, State Environmental, Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China.
与原始多环芳 (PAHs) 相比,化多环芳 (NPAHs) 导致更严重的氧化应激并加速动脉样硬化. NPAHs显著影响健康风险,突出其在风险评估中的重要性.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 心血管研究研究心血管研究
背景情况:
- 颗粒物2.5 (PM2.5) 是动脉样硬化的一个危险因素.
- 多环芳 (PAHs) 有助于PM2.5的毒性.
- 化PAH (NPAH) 具有强烈的氧化特性.
研究的目的:
- 为了比较子[a]皮林 (BaP) 和6-子子[a]皮林 (NBaP) 对动脉样硬化的作用.
- 评估替代剂在PAH引起的毒性中的作用.
- 评估NPAHs对内皮细胞和脂质代谢的影响.
主要方法:
- 在人静脉内皮细胞 (HUVEC) 的体外实验.
- 在体内实验中,ApoE-/-) 缺乏Apolipoprotein E (ApoE-/-) 的小鼠暴露在PM2.5结合的NBaP和BaP中.
- 血清和大动脉的生物化学分析,包括脂质样本和氧化应激标志物.
- 动脉样硬化斑块的组织学检查.
主要成果:
- 由于严重的氧化应激,NBaP暴露导致比BaP更大的HUVEC活力抑制和细胞损伤.
- 在ApoE-/-小鼠中,与PM2.5结合的NBaP诱导了脂质失调和氧化应激,导致动脉样硬化斑块的形成.
- BaP和对照组没有显示出显著的生理或病理变化.
结论:
- NPAHs通过严重的氧化应激诱导内皮功能障碍和脂质失调,加速动脉样硬化比PAHs更快.
- 替代剂显著加剧了多环芳香化合物的负面健康影响.
- 在对多环芳香化合物的健康风险评估中,NPAH是关键因素.
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