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通过调节miR-20a-5p/GGPPS1通路,CircUBR1 Knockdown可以缓解呼吸器引起的肺损伤
Li Wang1, Qiuqi Lin1, Benzhong Wei2
1Department of Respiratory and Critical Care Medicine, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210002, China.
Cellular signalling
|October 12, 2023
概括
循环RNA UBR1 (circUBR1) 通过调节GGPPS1.1来加剧呼吸器诱导的肺损伤 (VILI). 击败circUBR1可通过miR-20a-5p/GGPPS1通路缓解肺损伤和炎症,为VILI.提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 病理学 病理学 病理学
背景情况:
- 呼吸机引起的肺损伤 (VILI) 是机械通风的一个关键并发症.
- 循环RNAs (circRNAs) 在各种生物过程和疾病中发挥着新兴的作用.
- 在VILI中circUBR1的特定作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究circUBR1在VILI中的功能作用和潜在机制.
- 探索circUBR1作为VILI治疗的潜在治疗点.
主要方法:
- 在小鼠和小鼠膜上皮细胞中建立VILI模型.
- 定量实时聚合酶连锁反应 (qRT-PCR) 来评估circRNA和miRNA的表达.
- 西方斑块和免疫组织化学评估蛋白质水平 (GGPPS1).
- 组织病理学分析,细胞活力测定 (CCK-8) 和流细胞测量用于细胞亡评估.
- 使用ELISA和西班牙的炎症性细胞因子 (IL-1β,IL-18,IL-6,TNF-α) 的测量.
主要成果:
- 在VILI肺组织中,circUBR1和GGPPS1的表达显著上调,而miR-20a-5p则下调.
- 在VILI小鼠和细胞中,circUBR1 knockdown改善了肺损伤,减少了亡,并降低了炎症性细胞因子水平.
- circUBR1作为miR-20a-5p的分子海绵,GGPPS1被确定为miR-20a-5p的基因.
- 降低miR-20a-5p的调节或GGPPS1的过度表达逆转了circUBR1敲击的保护作用.
结论:
- 通过调节miR-20a-5p/GGPPS1通路,circUBR1 knockdown通过调节miR-20a-5p/GGPPS1通路来减轻VILI和相关炎症.
- circUBR1代表了一种有前途的治疗点,用于管理呼吸机引起的肺损伤.
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