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工程pH敏感的可侵蚀性奇托桑水凝复合物含有菌体:水凝和菌体之间的相互作用对抗金黄色葡萄球菌

Reuben Wang¹, Yu-Jia Yeh², Yu-Ning An²

¹Institute of Food Safety and Health, National Taiwan University, Taipei City, Taiwan; Master of Public Health (MPH) Program, National Taiwan University, Taipei City, Taiwan; GIP-TRIAD Master's Degree in Agro-Biomedical Science, National Taiwan University, Taipei City, Taiwan.

International journal of biological macromolecules

|October 12, 2023

概括

这项研究引入了一种新型的四分化奇托-化物-改性聚-酸-共聚-乙烯基醇水凝,用于增强菌体传递. 开发的冷解处理的菌体载水凝 (FTP) 显示了受控的菌体释放和强大的抗菌活性.

科学领域:

生物材料科学生物材料科学
药物输送系统药物输送系统
微生物学微生物学

背景情况:

菌体封装对于稳定性和可控剂量至关重要.
在对抗细菌感染方面,开发有效的菌体输送系统仍然是一个挑战.

研究的目的:

开发和描述一种用于菌体封装和输送的新型水凝.
评估菌体载水凝的抗菌疗效和释放动力学.

主要方法:

制造四分化奇托 (QCS) 和化物修饰的多 ((xylitol sebacate) -联合多 ((ethylene glycol) (APP) 水凝.
冷解 (FT) 处理以增强水凝的性能.
将菌体44AHJD封装到水凝 (FTP) 中.
评估水凝硬度,在不同温度和pH值下菌体释放动力学,以及生物相容性 (血液溶解试验).
对抗细菌和菌素耐药菌株的抗菌活性的评估.

主要成果:

FT处理增加了水凝的刚性 (10到20kPa) 和多孔性.
根据Korsmeyer-Peppas模型,FTP显示了菌素粒子的异常扩散.
观察到pH敏感的菌体释放,在中性pH下释放量更高.
证实了高生物相容性,血液溶解比率<2%.
显著的抗菌活性被证明,在10小时内释放了60%的菌体.

结论:

QCS-APP水凝,特别是在FT处理后,是菌体输送的有希望的平台.
开发的水凝系统提供受控的菌体释放和有效的抗菌作用对抗细菌和菌体耐药菌株.
这种方法有可能促进菌体治疗的应用.

关键词:

基托桑是一种酸盐.有控制释放的释放.对菌体有耐药性的

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