Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.3K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

CD4-directed nanoblades enable selective genome editing in CD4<sup>+</sup> cells and HIV suppression in vitro and in vivo.

EMBO molecular medicine·2026
Same author

Reference intervals reimagined with IRIS for earlier detection and better disease monitoring.

Scientific reports·2026
Same author

Vaginal host-microbe signatures linked to placental outcomes in mares.

Equine veterinary journal·2026
Same author

In ovo versus ex ovo incubation differentially shapes chorioallantoic membrane maturation, angiogenesis, and tumor growth.

Scientific reports·2026
Same author

HIV immunological non-responders show low SKAP1 concentration and DNA hypermethylation in the SKAP1 promotor region: Low Skap1 in HIV Immunological Non-Responders.

AIDS (London, England)·2026
Same author

Hemangiosarcoma: A Systematic Review of Biomarkers in Diagnosis, Prognosis, and Therapeutic Strategies.

Veterinary and comparative oncology·2026

相关实验视频

Updated: Jul 13, 2025

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

9.7K

用数字PCR测量DNA质量,使用概率计算.

David Gleerup1, Yao Chen2, Willem Van Snippenberg3

  • 1Laboratory of Veterinary Morphology, Ghent University, Belgium; Ghent University Digital PCR Consortium, Ghent University, Belgium.

Analytica chimica acta
|October 12, 2023
PubMed
概括

使用数字PCR (dPCR) 的一种新的基于概率的方法,可以在广泛的度范围内准确评估DNA完整性. 这种先进的技术克服了传统基于比率的方法的局限性,特别是在更高的DNA水平上,确保可靠的DNA质量分析.

关键词:
DNA的完整性 DNA的完整性数字PCR是数字的PCR.艾滋病病毒 艾滋病病毒 艾滋病病毒在 dPCR 中使用 dPCR.

更多相关视频

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

1.8K
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

10.9K

相关实验视频

Last Updated: Jul 13, 2025

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella
07:11

Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella

Published on: May 13, 2019

9.7K
Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

1.8K
Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format
05:58

Digital Polymerase Chain Reaction Assay for the Genetic Variation in a Sporadic Familial Adenomatous Polyposis Patient Using the Chip-in-a-tube Format

Published on: August 20, 2018

10.9K

科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 生物技术是生物技术.

背景情况:

  • 准确的DNA完整性评估对于各种生物分子应用至关重要.
  • 现有的多重数字PCR (dPCR) 方法用于DNA完整性分析,受到DNA度的限制.
  • 在dPCR中基于比例的传统计算仅在低DNA度时有效.

研究的目的:

  • 开发和验证一种新的基于概率的方法,用于使用dPCR进行DNA完整性分析.
  • 为了能够在DNA度的广泛动态范围内准确地评估DNA质量.
  • 扩展dPCR的多重复合能力,以提高DNA完整性的测量.

主要方法:

  • 开发了一种使用概率计算的多重数字PCR (dPCR) 方法.
  • 该方法在低度,中度和高度 (600,12500和30000副本/反应) 中使用人工双链DNA进行了测试.
  • 概率计算方程扩展到4个维度,用于概念验证的验证.

主要成果:

  • 提出的基于概率的方法与基于比率的方法相比,显示出更高的准确性,特别是在中间和高DNA度下.
  • 在12500副本/反应时,平均相对误差为16.7% (建议) 与20.7% (基于比率).
  • 在30000个副本/反应时,平均相对误差为9.3% (拟议) 与60.9% (基于比率),只有拟议的方法提供了准确的测量.
  • 提出的方法表现出一个恒定的偏差,而基于比率的方法的偏差随着DNA完整性而减少.
  • 概率方程的四维扩展在概念验证实验中显示出有效性.

结论:

  • 这种新的概率计算方法在DNA度的广泛动态范围内提供了准确可靠的DNA完整性分析.
  • 这种方法克服了数字PCR中基于比率的传统方法的局限性.
  • 将该方法扩展到4个复数增强了DNA完整度测量的准确性和灵活性.